User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/08/29: Difference between revisions
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| BSA||HAuCl4||BSA||HAuCl4||H2O [mL] | | BSA||HAuCl4||BSA [μL]||HAuCl4||H2O [mL] | ||
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| 1||60||253.07||147.93||3.5990 | | 1||60||253.07||147.93||3.5990 |
Revision as of 11:23, 11 September 2012
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Objective
Description
Data and Calculations
FW HAuCl4: 393.83 g/mol Theoretical Concentration of Stock: 10mM Volume of Stock: 25mL Theoretical Mass: 25mL × 10-3 L/mL × 10mM × 10-3 mol/mmol × 393.83 g/mol = 0.0985 g HAuCl4 Measured Mass: 0.0971 g HAuCl4 Calculated Concentration: 9862 μM 0.0971g × (1/393.83 g/mol) × (1/25E-3 L) × 106 μM/mol = 9862 μM
FW BSA: 66430 g/mol Theoretical Concentration of Stock: 15μM Volume of Stock: 10mL Theoretical Mass: 10mL × 10-3 L/mL × 15μM × 10-6 mol/mmol × 66430 g/mol = 0.00996 g BSA Measured Mass: 0.0105 g BSA Calculated Concentration: 15.806 μM 0.0105g × (1/66430 g/mol) × (1/10E-3 L) × 106 μM/mol = 15.806 μM
NotesThis area is for any observations or conclusions that you would like to note. |