User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/08/29: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|300px]]<span style="font-size:22px;"> AU Biomaterials Design Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
* Insert content here...




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__NOTOC__
==Objective==
*to make stock solutions of HAuCl<sub>4</sub> and BSA.
*to make 14 differently concentrated HAuCl<sub>4</sub> and BSA mixtures.
 
==Description==
# Calculations were made to determine how much HAuCl<sub>4</sub> was required to make 25mL of 10mM stock solution.
# Calculations were made to determine how much BSA was required to make 10mL of 15μM stock solution.
# The appropriate amounts of HAuCl<sub>4</sub> and BSA were massed separately and added to separate test tubes.
# The appropriate amount of H<sub>2</sub>O were added to each test tube. (25mL to the HAuCl<sub>4</sub> test tube and 10mL to the BSA test tube.)
# The test tubes were covered with parafilm.
# The test tube containing HAuCl<sub>4</sub> was also completely covered in aluminum foil.
# Then, using the m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub> equation, the amount of each stock solution required to make 4mL mixtures with the Au<sup>3+</sup>/BSA concentration ratio of 60μM, 80μM, 100μM, 120μM, 128μM, 130μM, 132μM, 133μM, 134μM, 136μM, 138μM, 140μM, 160μM, and 170μM.
# After the amounts of each stock solution was determined, the appropriate amounts of HAuCl<sub>4</sub>, BSA, and H<sub>2</sub>O were added to each test tube.
# The test tubes were capped, covered in aluminum foil, labeled, and placed in the oven at 85°C at 4 hours.
 
==Data and Calculations==
* '''HAuCl<sub>4</sub> Stock'''
  '''FW HAuCl<sub>4</sub>''': 393.83 g/mol
  '''Theoretical Concentration of Stock''': 10mM
  '''Volume of Stock''': 25mL
  '''Theoretical Mass''':
        25mL × 10<sup>-3</sup> L/mL × 10mM × 10<sup>-3</sup> mol/mmol × 393.83 g/mol = 0.0985 g HAuCl<sub>4</sub>
  '''Measured Mass''': 0.0971 g HAuCl<sub>4</sub>
  '''Calculated Concentration''': 9862 μM
        0.0971g × (1/393.83 g/mol) × (1/25E-3 L) × 10<sup>6</sup> μM/mol = 9862 μM
 
*'''BSA Stock'''
'''FW BSA''': 66430 g/mol
  '''Theoretical Concentration of Stock''': 15μM
  '''Volume of Stock''': 10mL
  '''Theoretical Mass''':
        10mL × 10<sup>-3</sup> L/mL × 15μM × 10<sup>-6</sup> mol/mmol × 66430 g/mol = 0.00996 g BSA
  '''Measured Mass''': 0.0105 g BSA
  '''Calculated Concentration''': 15.806 μM
        0.0105g × (1/66430 g/mol) × (1/10E-3 L) × 10<sup>6</sup> μM/mol = 15.806 μM
 
*'''Volumes of HAuCl<sub>4</sub>, BSA, H<sub>2</sub> required for various 4mL HAuCl<sub>4</sub>/BSA mixtures'''
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''uM Ratio [Au/BSA]'''
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''Volume [uL]'''
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|''''''
|-
| BSA||HAuCl4||BSA [μL]||HAuCl4||H2O [mL]
|-
| 1||60||253.07||147.93||3.5990
|-
| 1||80||253.07||197.24||3.5497
|-
| 1||100||253.07||246.55||3.5004
|-
| 1||120||253.07||295.86||3.4511
|-
| 1||128||253.07||315.58||3.4313
|-
| 1||130||253.07||320.52||3.4264
|-
| 1||132||253.07||325.45||3.4215
|-
| 1||133||253.07||327.91||3.4190
|-
| 1||134||253.07||330.38||3.4166
|-
| 1||136||253.07||335.31||3.4116
|-
| 1||138||253.07||340.24||3.4067
|-
| 1||140||253.07||345.17||3.4018
|-
| 1||160||253.07||394.48||3.3525
|-
| 1||170||253.07||419.14||3.3278
|-
|
|-
| ||||||4341.75||Total uL
|}
 
==Notes==
*The stocks were made with the incorrect concentrations, so the reaction did not occur properly.
*The stocks were made without the parafilm protection on the spatula.
 
==Image of the Solutions Produced==
 
[[Image:All.jpg]]
*All the solutions from August 29, ranging from 60μM to 170μM Au/BSA concentration. The solution was made without the parafilmed spatula. THe solution spent 4 hours in a 85°C oven.

Revision as of 12:14, 12 September 2012

AU Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

  • to make stock solutions of HAuCl4 and BSA.
  • to make 14 differently concentrated HAuCl4 and BSA mixtures.

Description

  1. Calculations were made to determine how much HAuCl4 was required to make 25mL of 10mM stock solution.
  2. Calculations were made to determine how much BSA was required to make 10mL of 15μM stock solution.
  3. The appropriate amounts of HAuCl4 and BSA were massed separately and added to separate test tubes.
  4. The appropriate amount of H2O were added to each test tube. (25mL to the HAuCl4 test tube and 10mL to the BSA test tube.)
  5. The test tubes were covered with parafilm.
  6. The test tube containing HAuCl4 was also completely covered in aluminum foil.
  7. Then, using the m1v1=m2v2 equation, the amount of each stock solution required to make 4mL mixtures with the Au3+/BSA concentration ratio of 60μM, 80μM, 100μM, 120μM, 128μM, 130μM, 132μM, 133μM, 134μM, 136μM, 138μM, 140μM, 160μM, and 170μM.
  8. After the amounts of each stock solution was determined, the appropriate amounts of HAuCl4, BSA, and H2O were added to each test tube.
  9. The test tubes were capped, covered in aluminum foil, labeled, and placed in the oven at 85°C at 4 hours.

Data and Calculations

  • HAuCl4 Stock
  FW HAuCl4: 393.83 g/mol
  Theoretical Concentration of Stock: 10mM
  Volume of Stock: 25mL
  Theoretical Mass:
        25mL × 10-3 L/mL × 10mM × 10-3 mol/mmol × 393.83 g/mol = 0.0985 g HAuCl4
  Measured Mass: 0.0971 g HAuCl4
  Calculated Concentration: 9862 μM
        0.0971g × (1/393.83 g/mol) × (1/25E-3 L) × 106 μM/mol = 9862 μM
  • BSA Stock
FW BSA: 66430 g/mol
  Theoretical Concentration of Stock: 15μM
  Volume of Stock: 10mL
  Theoretical Mass:
        10mL × 10-3 L/mL × 15μM × 10-6 mol/mmol × 66430 g/mol = 0.00996 g BSA
  Measured Mass: 0.0105 g BSA
  Calculated Concentration: 15.806 μM
        0.0105g × (1/66430 g/mol) × (1/10E-3 L) × 106 μM/mol = 15.806 μM
  • Volumes of HAuCl4, BSA, H2 required for various 4mL HAuCl4/BSA mixtures
uM Ratio [Au/BSA] ' Volume [uL] ' '
BSA HAuCl4 BSA [μL] HAuCl4 H2O [mL]
1 60 253.07 147.93 3.5990
1 80 253.07 197.24 3.5497
1 100 253.07 246.55 3.5004
1 120 253.07 295.86 3.4511
1 128 253.07 315.58 3.4313
1 130 253.07 320.52 3.4264
1 132 253.07 325.45 3.4215
1 133 253.07 327.91 3.4190
1 134 253.07 330.38 3.4166
1 136 253.07 335.31 3.4116
1 138 253.07 340.24 3.4067
1 140 253.07 345.17 3.4018
1 160 253.07 394.48 3.3525
1 170 253.07 419.14 3.3278
4341.75 Total uL

Notes

  • The stocks were made with the incorrect concentrations, so the reaction did not occur properly.
  • The stocks were made without the parafilm protection on the spatula.

Image of the Solutions Produced

  • All the solutions from August 29, ranging from 60μM to 170μM Au/BSA concentration. The solution was made without the parafilmed spatula. THe solution spent 4 hours in a 85°C oven.
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