User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/04: Difference between revisions

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==Objective==
==Objective==


*to run UV-Vis spectra on the Au/BSA mixtures that Dr. Miller made on [http://openwetware.org/wiki/User:Abigail_E._Miller/Notebook/CHEM571_Lab_Project/2012/08/31 Friday],
*to run UV-Vis spectra on the Au/BSA mixtures that Dr. Miller made on [[User:Abigail_E._Miller/Notebook/CHEM571_Lab_Project/2012/08/31| Friday]],
*to centrifuge the mixtures that Dr. Miller made,
*to centrifuge the mixtures that Dr. Miller made,
*to make 100mM Tris Buffer with pH 10 and pH 8, and
*to make 100mM Tris Buffer with pH 10 and pH 8, and
*to remake Au/BSA mixtures with concentrations of 60-133 because no purple solutions were created in the other mixtures.
*to remake Au/BSA mixtures with concentrations of 60-133 because no purple solutions were created in the other mixtures.


==Description==
==Description==


*'''UV-Vis'''
*'''UV-Vis'''
1. UV-Vis was run on 1mL samples of water and each sample with the wavelength range of 700-200nm, looking for a peak around 550nm.
#UV-Vis was run on 1mL samples of water and each sample with the wavelength range of 700-200nm, looking for a peak around 550nm.
2. The data points were collected and imported in Excel.
#The data points were collected and imported in Excel.
3. The data was corrected for water and plotted to better visualize the absorbance.  
#The data was corrected for water and plotted to better visualize the absorbance.  


*[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/04 Description of Centrifuge and Observations]
*'''[[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/04| Description of Centrifuge and Observations]]'''


*[User:Melissa_Novy/Notebook/CHEM-571/2012/09/04 Preparation of Tris Buffer]
*'''[[User:Melissa_Novy/Notebook/CHEM-571/2012/09/04| Preparation of Tris Buffer]]'''


*The Au/BSA solutions were remade using the same protocol from [User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/08/29 last week], except that the metal spatula was wrapped in parafilm so that the metal from the spatula would not react with the gold. Concentrations of 60, 80, 100, 120, 128, 130, 132, and 133 were made.  
*'''Remaking of the Au/BSA Solutons'''
#The Au/BSA solutions were remade using the same protocol from [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/08/29| last week]], except that the metal spatula was wrapped in parafilm so that the metal from the spatula would not react with the gold. Concentrations of 60, 80, 100, 120, 128, 130, 132, and 133 were made.


==Data and Calculations==
==Data and Calculations==


N/A
*'''Table of Measurements for Au/BSA mixtures'''
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Stock Solution Concentrations'''
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|''''''
|-
| Theoretical||||||Actual [uM]||
|-
| BSA||HAuCl4||||BSA||HAuCl4
|-
| 15 uM||10 mM||||15.806||9862
|}
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Vol [uL]'''
| align="center" style="background:#f0f0f0;"|''''''
|-
| BSA||HAuCl4
|-
| 4000||4000
|}
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''uM Ratio [Au/BSA]'''
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''Volume [uL]'''
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|''''''
|-
| BSA||HAuCl4||BSA [μL]||HAuCl4||H2O [mL]
|-
| 1||60||253.07||147.93||3.5990
|-
| 1||80||253.07||197.24||3.5497
|-
| 1||100||253.07||246.55||3.5004
|-
| 1||120||253.07||295.86||3.4511
|-
| 1||128||253.07||315.58||3.4313
|-
| 1||130||253.07||320.52||3.4264
|-
| 1||132||253.07||325.45||3.4215
|-
| 1||133||253.07||327.91||3.4190
|-
| 1||134||253.07||330.38||3.4166
|-
| 1||136||253.07||335.31||3.4116
|-
| 1||138||253.07||340.24||3.4067
|-
| 1||140||253.07||345.17||3.4018
|-
| 1||160||253.07||394.48||3.3525
|-
| 1||170||253.07||419.14||3.3278
|-
| ||||||4341.75||Total uL
|}


==Notes==
==Notes==
Only the mixture with concentration of 80 Au/BSA was a homologous purple mixture.
*Only the mixture with concentration of 80 Au/BSA was a homologous purple mixture. [made 8/30]
*The stocks made today were made with the incorrect concentrations, so the reaction did not occur properly.
 
==Images from the Solutions made by Dr. Miller==
 
[[Image:Dr_Miller_Solutions.jpg]]
*All the solutions from Dr Miller's batch from August 30. The solution was made with the parafilmed spatula. The solution spent 4 hours in a 85°C oven.
 
 
==Image of Solution made on September 4th==
 
[[Image:IMAG0730.jpg]]
*All the solutions looked identical. The reactants did not react with each other, and the concentration ratios were off significantly.

Revision as of 11:54, 12 September 2012

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Objective

  • to run UV-Vis spectra on the Au/BSA mixtures that Dr. Miller made on Friday,
  • to centrifuge the mixtures that Dr. Miller made,
  • to make 100mM Tris Buffer with pH 10 and pH 8, and
  • to remake Au/BSA mixtures with concentrations of 60-133 because no purple solutions were created in the other mixtures.

Description

  • UV-Vis
  1. UV-Vis was run on 1mL samples of water and each sample with the wavelength range of 700-200nm, looking for a peak around 550nm.
  2. The data points were collected and imported in Excel.
  3. The data was corrected for water and plotted to better visualize the absorbance.
  • Remaking of the Au/BSA Solutons
  1. The Au/BSA solutions were remade using the same protocol from last week, except that the metal spatula was wrapped in parafilm so that the metal from the spatula would not react with the gold. Concentrations of 60, 80, 100, 120, 128, 130, 132, and 133 were made.

Data and Calculations

  • Table of Measurements for Au/BSA mixtures
Stock Solution Concentrations ' ' ' '
Theoretical Actual [uM]
BSA HAuCl4 BSA HAuCl4
15 uM 10 mM 15.806 9862
Vol [uL] '
BSA HAuCl4
4000 4000
uM Ratio [Au/BSA] ' Volume [uL] ' '
BSA HAuCl4 BSA [μL] HAuCl4 H2O [mL]
1 60 253.07 147.93 3.5990
1 80 253.07 197.24 3.5497
1 100 253.07 246.55 3.5004
1 120 253.07 295.86 3.4511
1 128 253.07 315.58 3.4313
1 130 253.07 320.52 3.4264
1 132 253.07 325.45 3.4215
1 133 253.07 327.91 3.4190
1 134 253.07 330.38 3.4166
1 136 253.07 335.31 3.4116
1 138 253.07 340.24 3.4067
1 140 253.07 345.17 3.4018
1 160 253.07 394.48 3.3525
1 170 253.07 419.14 3.3278
4341.75 Total uL

Notes

  • Only the mixture with concentration of 80 Au/BSA was a homologous purple mixture. [made 8/30]
  • The stocks made today were made with the incorrect concentrations, so the reaction did not occur properly.

Images from the Solutions made by Dr. Miller

  • All the solutions from Dr Miller's batch from August 30. The solution was made with the parafilmed spatula. The solution spent 4 hours in a 85°C oven.


Image of Solution made on September 4th

  • All the solutions looked identical. The reactants did not react with each other, and the concentration ratios were off significantly.
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