User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/05: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|300px]]<span style="font-size:22px;"> AU Biomaterials Design Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
==Objectives==
* Insert content here...
 
*to re-make the Au/BSA solutions, because the mixtures from yesterday did not react,
*to re-suspend the fibers in varying concentrations of Tris Buffer with pHs 10 and 8, and
*to run UV-vis on the resuspended fibers.
 
==Description==
 
*[[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/08/29| Re-making Au/BSA solutions]]
Adjustments:
#The spatula was wrapped in parafilm to measure out both the BSA and the HAuCl<sub>4</sub>.
#Only one concentration was made initially as a test. It was placed in the oven at 85°C for an hour to make sure a reaction was taking place. This TEST sample was removed after an hour. Dark Blue/Purple fibers formed. This TEST sample was saved for later testing.  
#After it was confirmed that a reaction was indeed taking place, the mixtures were made with concentrations of 60μM, 80μM, 100μM, 120μM, 128μM, 130μM, 132μM, 133μM, 134μM, 136μM, 138μM, 140μM, 160μM, and 170μM, taking into account the new masses and new actual concentrations of the stock solutions.  
 
*[[User:Melissa_Novy/Notebook/CHEM-571/2012/09/05| Re-suspending Fibers]]
 
*[[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/09/04| UV-vis]]
Adjustments:
#1mL of the resuspended fibers were examined.  
 
==Data==
 
*'''Table of Measurements for Au/BSA mixtures'''
 
 
 
*'''UV-Vis of Resuspended Fibers'''


==Notes==


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Revision as of 12:24, 5 September 2012

AU Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objectives

  • to re-make the Au/BSA solutions, because the mixtures from yesterday did not react,
  • to re-suspend the fibers in varying concentrations of Tris Buffer with pHs 10 and 8, and
  • to run UV-vis on the resuspended fibers.

Description

Adjustments:

  1. The spatula was wrapped in parafilm to measure out both the BSA and the HAuCl4.
  2. Only one concentration was made initially as a test. It was placed in the oven at 85°C for an hour to make sure a reaction was taking place. This TEST sample was removed after an hour. Dark Blue/Purple fibers formed. This TEST sample was saved for later testing.
  3. After it was confirmed that a reaction was indeed taking place, the mixtures were made with concentrations of 60μM, 80μM, 100μM, 120μM, 128μM, 130μM, 132μM, 133μM, 134μM, 136μM, 138μM, 140μM, 160μM, and 170μM, taking into account the new masses and new actual concentrations of the stock solutions.

Adjustments:

  1. 1mL of the resuspended fibers were examined.

Data

  • Table of Measurements for Au/BSA mixtures


  • UV-Vis of Resuspended Fibers

Notes


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