User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/05: Difference between revisions
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*[[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/08/29| Re-making Au/BSA solutions]] | *[[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/08/29| Re-making Au/BSA solutions]] | ||
**After the Au/BSA solutions were remade, UV-Vis was run on the samples to measure their absorbance. | |||
Adjustments: | Adjustments: | ||
#The spatula was wrapped in parafilm to measure out both the BSA and the HAuCl<sub>4</sub>. | #The spatula was wrapped in parafilm to measure out both the BSA and the HAuCl<sub>4</sub>. | ||
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#After it was confirmed that a reaction was indeed taking place, the mixtures were made with concentrations of 60μM, 80μM, 100μM, 120μM, 128μM, 130μM, 132μM, 133μM, 134μM, 136μM, 138μM, 140μM, 160μM, and 170μM, taking into account the new masses and new actual concentrations of the stock solutions. | #After it was confirmed that a reaction was indeed taking place, the mixtures were made with concentrations of 60μM, 80μM, 100μM, 120μM, 128μM, 130μM, 132μM, 133μM, 134μM, 136μM, 138μM, 140μM, 160μM, and 170μM, taking into account the new masses and new actual concentrations of the stock solutions. | ||
*[[User:Melissa_Novy/Notebook/CHEM-571/2012/09/05| | *Re-suspending Fibers | ||
**Please refer to [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/05| Melissa's entry]] for details on centrifuging the Au/BSA solutions and adding the Tris buffer. | |||
***2 different pHs of Tris buffer were tested: pH 8 and 10. | |||
***The concentrations of each pH of Tris buffer was observed using 1 mM, 100 μM, and 10 μM Tris buffer on fibers with arbitrary AU/BSA molar ratios. | |||
*[[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/09/04| UV-vis]] | *[[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/09/04| UV-vis]] | ||
**UV-Vis was run on 1mL of the resuspended fibers, with the wavelength range of 700-200nm, looking for a peak around 550nm. | |||
**The data was plotted on an Absorbance vs. Wavelength graph. | |||
==Data== | ==Data== | ||
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*'''UV-Vis of Resuspended Fibers via Tris Buffer''' | *'''UV-Vis of Resuspended Fibers via Tris Buffer''' | ||
**Please refer to [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/05|Melissa's Entry]] on UV-Vis Analysis to see the graph of Absorbance of AuNP/BSA fibers at various concentrations, resuspended in tris buffer at pH 8 and 10. | |||
**This graph shows the varying absorbances at 545nm, base on the buffer's acidity and concentration. | |||
==Notes== | ==Notes== |
Revision as of 10:39, 2 October 2012
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Objectives
Description
Adjustments:
Data
Notes
Image of solutions made September 5th
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