User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/19 - Revision history

Dhea Patel: /* Data */

2012-11-28T19:18:35Z

Data

←Older revision		Revision as of 19:18, 28 November 2012
Line 37:		Line 37:

	*This graph depicts the data obtained from 3 samples during fluorescence at 510nm over about 100 seconds. The three samples that ran contained 18mM 4-iodophenol and 2.3uM horseradish peroxidase with varying concentrations of luminol and hydrogen peroxide (1.25mM luminol and 1.7mM H<sub>2</sub>O<sub>2</sub>, 625uM luminol and 1.7mM H<sub>2</sub>O<sub>2</sub>, and 625uM luminol and 850uM H<sub>2</sub>O<sub>2</sub>).		*This graph depicts the data obtained from 3 samples during fluorescence at 510nm over about 100 seconds. The three samples that ran contained 18mM 4-iodophenol and 2.3uM horseradish peroxidase with varying concentrations of luminol and hydrogen peroxide (1.25mM luminol and 1.7mM H<sub>2</sub>O<sub>2</sub>, 625uM luminol and 1.7mM H<sub>2</sub>O<sub>2</sub>, and 625uM luminol and 850uM H<sub>2</sub>O<sub>2</sub>).
-	**The graph indicates that no significant difference in the reaction occurs when the concentration of Luminol is halved. The third curve with 625μM Luminol and 850μM hydrogen peroxide indicates that a significant loss in enzymatic activity occurs when the concentration of hydrogen peroxide is halved. However, this data needs to be supplemented with intermediate concentrations of Luminol and hydrogen peroxide to ensure that the observations hold true for varying concentration ratios.	+	*The graph indicates that no significant difference in the reaction occurs when the concentration of Luminol is halved. The third curve with 625μM Luminol and 850μM hydrogen peroxide indicates that a significant loss in enzymatic activity occurs when the concentration of hydrogen peroxide is halved. However, this data needs to be supplemented with intermediate concentrations of Luminol and hydrogen peroxide to ensure that the observations hold true for varying concentration ratios.

Dhea Patel: /* Data */

2012-11-28T19:18:20Z

Data

←Older revision		Revision as of 19:18, 28 November 2012
Line 34:		Line 34:
	==Data==		==Data==

-	[[Image:~~Fluorescence_at_510nm_versus_time~~-s-.png]]	+	[[Image:Fluorescence at 510nm versus time-s-.png\|650px]]
		+
	*This graph depicts the data obtained from 3 samples during fluorescence at 510nm over about 100 seconds. The three samples that ran contained 18mM 4-iodophenol and 2.3uM horseradish peroxidase with varying concentrations of luminol and hydrogen peroxide (1.25mM luminol and 1.7mM H<sub>2</sub>O<sub>2</sub>, 625uM luminol and 1.7mM H<sub>2</sub>O<sub>2</sub>, and 625uM luminol and 850uM H<sub>2</sub>O<sub>2</sub>).		*This graph depicts the data obtained from 3 samples during fluorescence at 510nm over about 100 seconds. The three samples that ran contained 18mM 4-iodophenol and 2.3uM horseradish peroxidase with varying concentrations of luminol and hydrogen peroxide (1.25mM luminol and 1.7mM H<sub>2</sub>O<sub>2</sub>, 625uM luminol and 1.7mM H<sub>2</sub>O<sub>2</sub>, and 625uM luminol and 850uM H<sub>2</sub>O<sub>2</sub>).
	**The graph indicates that no significant difference in the reaction occurs when the concentration of Luminol is halved. The third curve with 625μM Luminol and 850μM hydrogen peroxide indicates that a significant loss in enzymatic activity occurs when the concentration of hydrogen peroxide is halved. However, this data needs to be supplemented with intermediate concentrations of Luminol and hydrogen peroxide to ensure that the observations hold true for varying concentration ratios.		**The graph indicates that no significant difference in the reaction occurs when the concentration of Luminol is halved. The third curve with 625μM Luminol and 850μM hydrogen peroxide indicates that a significant loss in enzymatic activity occurs when the concentration of hydrogen peroxide is halved. However, this data needs to be supplemented with intermediate concentrations of Luminol and hydrogen peroxide to ensure that the observations hold true for varying concentration ratios.

Dhea Patel: /* Data */

2012-11-28T19:17:42Z

Data

←Older revision		Revision as of 19:17, 28 November 2012
Line 34:		Line 34:
	==Data==		==Data==

-	*Please refer to [[~~User~~:~~Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/19 \| Kay'~~s ~~entry~~]] ~~for the~~ graph ~~produced from~~ the data obtained from 3 samples during fluorescence at 510nm over about 100 seconds. The three samples that ran contained 18mM 4-iodophenol and 2.3uM horseradish peroxidase with varying concentrations of luminol and hydrogen peroxide (1.25mM luminol and 1.7mM H<sub>2</sub>O<sub>2</sub>, 625uM luminol and 1.7mM H<sub>2</sub>O<sub>2</sub>, and 625uM luminol and 850uM H<sub>2</sub>O<sub>2</sub>).	+	[[Image:Fluorescence_at_510nm_versus_time-s-.png]]
		+	*This graph depicts the data obtained from 3 samples during fluorescence at 510nm over about 100 seconds. The three samples that ran contained 18mM 4-iodophenol and 2.3uM horseradish peroxidase with varying concentrations of luminol and hydrogen peroxide (1.25mM luminol and 1.7mM H<sub>2</sub>O<sub>2</sub>, 625uM luminol and 1.7mM H<sub>2</sub>O<sub>2</sub>, and 625uM luminol and 850uM H<sub>2</sub>O<sub>2</sub>).
	**The graph indicates that no significant difference in the reaction occurs when the concentration of Luminol is halved. The third curve with 625μM Luminol and 850μM hydrogen peroxide indicates that a significant loss in enzymatic activity occurs when the concentration of hydrogen peroxide is halved. However, this data needs to be supplemented with intermediate concentrations of Luminol and hydrogen peroxide to ensure that the observations hold true for varying concentration ratios.		**The graph indicates that no significant difference in the reaction occurs when the concentration of Luminol is halved. The third curve with 625μM Luminol and 850μM hydrogen peroxide indicates that a significant loss in enzymatic activity occurs when the concentration of hydrogen peroxide is halved. However, this data needs to be supplemented with intermediate concentrations of Luminol and hydrogen peroxide to ensure that the observations hold true for varying concentration ratios.

-	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 20:30, 7 October 2012 (EDT)''':you need ot include graphs to support your conclusions and therefore the concentrations of the reactants.

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Dhea Patel: /* Description */

2012-11-28T19:16:51Z

Description

←Older revision		Revision as of 19:16, 28 November 2012
Line 21:		Line 21:
	==Description==		==Description==

-	*The stock solutions of HRP, H<sub>2</sub>O<sub>2</sub>, and phenol that were made [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/09/18\| yesterday]] were mixed and run under the fluorometer with an emission at ~~425nm~~.	+	*The stock solutions of HRP, H<sub>2</sub>O<sub>2</sub>, and phenol that were made [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/09/18\| yesterday]] were mixed and run under the fluorometer with an emission at 430nm.
	*10mL of 2.5mM Luminol stock was prepared using the following calculations:		*10mL of 2.5mM Luminol stock was prepared using the following calculations:
	**MW luminol: 177.16 g/mol		**MW luminol: 177.16 g/mol

Dhea Patel: /* Description */

2012-11-28T19:14:30Z

Description

←Older revision		Revision as of 19:14, 28 November 2012
Line 21:		Line 21:
	==Description==		==Description==

-	*The stock solutions of HRP, H<sub>2</sub>O<sub>2</sub>, and phenol that were made [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/09/18\| yesterday]] were mixed and run under the fluorometer ~~at 510nm.~~	+	*The stock solutions of HRP, H<sub>2</sub>O<sub>2</sub>, and phenol that were made [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/09/18\| yesterday]] were mixed and run under the fluorometer with an emission at 425nm.
-	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 20:29, 7 October 2012 (EDT)''':these were not run at 510nm. emission is at 425nm ~~or htat vicinity~~.	+
	*10mL of 2.5mM Luminol stock was prepared using the following calculations:		*10mL of 2.5mM Luminol stock was prepared using the following calculations:
	**MW luminol: 177.16 g/mol		**MW luminol: 177.16 g/mol

Dhea Patel: /* Objective */

2012-11-28T19:12:42Z

Objective

←Older revision		Revision as of 19:12, 28 November 2012
Line 17:		Line 17:
	==Objective==		==Objective==

-	*To determine enzymatic activity of horseradish peroxidase ~~via the usage of fluorometer under 510nm.~~	+	*To determine enzymatic activity of horseradish peroxidase via a luminol chemiluminescence assay measured with a fluorimeter.
-		+
-	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 20:27, 7 October 2012 (EDT)''':via a luminol chemiluminescence assay measured with a fluorimeter~~(not via a fluorimeter under 510nm, what does that mean?)~~	+

	==Description==		==Description==

Abigail E. Miller: /* Data */

2012-10-08T00:30:38Z

Data

←Older revision		Revision as of 00:30, 8 October 2012
Line 39:		Line 39:
	*Please refer to [[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/19 \| Kay's entry]] for the graph produced from the data obtained from 3 samples during fluorescence at 510nm over about 100 seconds. The three samples that ran contained 18mM 4-iodophenol and 2.3uM horseradish peroxidase with varying concentrations of luminol and hydrogen peroxide (1.25mM luminol and 1.7mM H<sub>2</sub>O<sub>2</sub>, 625uM luminol and 1.7mM H<sub>2</sub>O<sub>2</sub>, and 625uM luminol and 850uM H<sub>2</sub>O<sub>2</sub>).		*Please refer to [[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/19 \| Kay's entry]] for the graph produced from the data obtained from 3 samples during fluorescence at 510nm over about 100 seconds. The three samples that ran contained 18mM 4-iodophenol and 2.3uM horseradish peroxidase with varying concentrations of luminol and hydrogen peroxide (1.25mM luminol and 1.7mM H<sub>2</sub>O<sub>2</sub>, 625uM luminol and 1.7mM H<sub>2</sub>O<sub>2</sub>, and 625uM luminol and 850uM H<sub>2</sub>O<sub>2</sub>).
	**The graph indicates that no significant difference in the reaction occurs when the concentration of Luminol is halved. The third curve with 625μM Luminol and 850μM hydrogen peroxide indicates that a significant loss in enzymatic activity occurs when the concentration of hydrogen peroxide is halved. However, this data needs to be supplemented with intermediate concentrations of Luminol and hydrogen peroxide to ensure that the observations hold true for varying concentration ratios.		**The graph indicates that no significant difference in the reaction occurs when the concentration of Luminol is halved. The third curve with 625μM Luminol and 850μM hydrogen peroxide indicates that a significant loss in enzymatic activity occurs when the concentration of hydrogen peroxide is halved. However, this data needs to be supplemented with intermediate concentrations of Luminol and hydrogen peroxide to ensure that the observations hold true for varying concentration ratios.
		+
		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 20:30, 7 October 2012 (EDT)''':you need ot include graphs to support your conclusions and therefore the concentrations of the reactants.

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Abigail E. Miller: /* Description */

2012-10-08T00:29:49Z

Description

←Older revision		Revision as of 00:29, 8 October 2012
Line 24:		Line 24:

	*The stock solutions of HRP, H<sub>2</sub>O<sub>2</sub>, and phenol that were made [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/09/18\| yesterday]] were mixed and run under the fluorometer at 510nm.		*The stock solutions of HRP, H<sub>2</sub>O<sub>2</sub>, and phenol that were made [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/09/18\| yesterday]] were mixed and run under the fluorometer at 510nm.
		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 20:29, 7 October 2012 (EDT)''':these were not run at 510nm. emission is at 425nm or htat vicinity.
	*10mL of 2.5mM Luminol stock was prepared using the following calculations:		*10mL of 2.5mM Luminol stock was prepared using the following calculations:
	**MW luminol: 177.16 g/mol		**MW luminol: 177.16 g/mol

Abigail E. Miller: /* Objective */

2012-10-08T00:27:54Z

Objective

←Older revision		Revision as of 00:27, 8 October 2012
Line 18:		Line 18:

	*To determine enzymatic activity of horseradish peroxidase via the usage of fluorometer under 510nm.		*To determine enzymatic activity of horseradish peroxidase via the usage of fluorometer under 510nm.
		+
		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 20:27, 7 October 2012 (EDT)''':via a luminol chemiluminescence assay measured with a fluorimeter(not via a fluorimeter under 510nm, what does that mean?)

	==Description==		==Description==

Dhea Patel: /* Data */

2012-10-02T18:34:52Z

Data

←Older revision		Revision as of 18:34, 2 October 2012
Line 35:		Line 35:

	*Please refer to [[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/19 \| Kay's entry]] for the graph produced from the data obtained from 3 samples during fluorescence at 510nm over about 100 seconds. The three samples that ran contained 18mM 4-iodophenol and 2.3uM horseradish peroxidase with varying concentrations of luminol and hydrogen peroxide (1.25mM luminol and 1.7mM H<sub>2</sub>O<sub>2</sub>, 625uM luminol and 1.7mM H<sub>2</sub>O<sub>2</sub>, and 625uM luminol and 850uM H<sub>2</sub>O<sub>2</sub>).		*Please refer to [[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/19 \| Kay's entry]] for the graph produced from the data obtained from 3 samples during fluorescence at 510nm over about 100 seconds. The three samples that ran contained 18mM 4-iodophenol and 2.3uM horseradish peroxidase with varying concentrations of luminol and hydrogen peroxide (1.25mM luminol and 1.7mM H<sub>2</sub>O<sub>2</sub>, 625uM luminol and 1.7mM H<sub>2</sub>O<sub>2</sub>, and 625uM luminol and 850uM H<sub>2</sub>O<sub>2</sub>).
-		+	**The graph indicates that no significant difference in the reaction occurs when the concentration of Luminol is halved. The third curve with 625μM Luminol and 850μM hydrogen peroxide indicates that a significant loss in enzymatic activity occurs when the concentration of hydrogen peroxide is halved. However, this data needs to be supplemented with intermediate concentrations of Luminol and hydrogen peroxide to ensure that the observations hold true for varying concentration ratios.

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->