User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/26

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Objective

In the morning (~8:30am)

  • to prepare the cells for protein expression
  • to make IPTG

Description

Prepared in the Morning

*Preparing the Cells for Protein Expression

  1. The starter cultures were centrifuged at 4500rpm for 15 minutes.
  2. The cell pellets were resuspended in 4mL of fresh LB (from one of the Fernbach flasks)
  3. The expression culture media was inoculated by dividing the resuspended cells among the Fernbach flasks.
    • The aluminum covering the Fernbach flasks were re-sterilized before recovering the flasks.
  4. The expression cultures were incubated at 37°C and 160rpm.

*Making IPTG


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