User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/10/09

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(Autocreate 2012/10/09 Entry for User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook)
Current revision (10:10, 25 October 2012) (view source)
 
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==Objective==
==Objective==
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#To make biocompatible protein films with specific reactive sites
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*To run atomic absorbance on Au/BSA samples
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#Explore the solution conditions (pH, ionic strength, buffer, stabilizers)  that produce stable protein – gold nanoparticle suspensions
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==Description==
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[[User:Melissa_Novy/Notebook/CHEM-571/2012/10/09| Please refer to Melissa's entry for further details on running Au/BSA mixtures through the AAS]]
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*Because the fibers were mixed and floating in the solution, some of the Au/BSA mixtures were filtered using 450nm pored-filter paper.
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**The solutions that were filtered turned from a pinkish color (indicating the presence of gold) to a colorless solution.
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*All the mixtures were run through the AA.
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*The AA used 7 standards as controls.
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*The AA tubing was placed in the sample tube and allowed to burn the sample until a steady absorbance was obtained. Then, the tube was placed in de-ionized water until the next sample was ready to be run.
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*As predicted, the filtered mixtures showed little to no gold particles present in the solution, which means that the gold was filtered out along with the fibers.

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Objective

  • To run atomic absorbance on Au/BSA samples

Description

Please refer to Melissa's entry for further details on running Au/BSA mixtures through the AAS

  • Because the fibers were mixed and floating in the solution, some of the Au/BSA mixtures were filtered using 450nm pored-filter paper.
    • The solutions that were filtered turned from a pinkish color (indicating the presence of gold) to a colorless solution.
  • All the mixtures were run through the AA.
  • The AA used 7 standards as controls.
  • The AA tubing was placed in the sample tube and allowed to burn the sample until a steady absorbance was obtained. Then, the tube was placed in de-ionized water until the next sample was ready to be run.
  • As predicted, the filtered mixtures showed little to no gold particles present in the solution, which means that the gold was filtered out along with the fibers.



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