User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/10/17

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2012/10/17 Entry for User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook)
Line 17: Line 17:
==Objective==
==Objective==
-
#To make biocompatible protein films with specific reactive sites
+
* to prepare and run RuATP column.
-
#Explore the solution conditions (pH, ionic strength, buffer, stabilizers)  that produce stable protein – gold nanoparticle suspensions
+
 
 +
==Description==
 +
*1.5L of 2:4:4:3 isopropanol:dioxane:water:25% ammonium hydride solution was made.
 +
*a [[User:Dhea_Patel/Notebook/Phosphorylation/2012/06/25| silica gel column]] was made to purify the RuATP. The isopropanol:dioxane:water:25% ammonium hydride was used as the solvent instead of acetonitrile. No variations in the solvent were used.
 +
 
 +
* purified Ru was dissolved in about 10mL of DMF before added to the ATP reaction.

Revision as of 13:02, 17 October 2012

Search this Project

Customize your entry pages

Objective

  • to prepare and run RuATP column.

Description

  • 1.5L of 2:4:4:3 isopropanol:dioxane:water:25% ammonium hydride solution was made.
  • a silica gel column was made to purify the RuATP. The isopropanol:dioxane:water:25% ammonium hydride was used as the solvent instead of acetonitrile. No variations in the solvent were used.
  • purified Ru was dissolved in about 10mL of DMF before added to the ATP reaction.



Personal tools