User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/10/24

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(Autocreate 2012/10/24 Entry for User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook)
(Objective)
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==Objective==
==Objective==
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#To make biocompatible protein films with specific reactive sites
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*to run Fluorescence on Luminol-HRP assays.
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#Explore the solution conditions (pH, ionic strength, buffer, stabilizers)  that produce stable protein – gold nanoparticle suspensions
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==Description==
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Please refer to table for volumes and concentrations of HRP, Luminol, Phenol, Buffer, and hydrogen peroxide used.
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[[Image:20121024.png]]
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==Data==
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[[Image:Trail_6.png]]
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*All the trails, except trial 6 had a straight peak, showing do drop off of the fluorescence. Trail 6, containing, 454uL 0.564mM Luminol, 112.7uL 5mM hydrogen peroxide, 16.5uL 0.9uM HRP, 13.2 uL 18mM 4-Iodophenol, and 1000uL Carbonate Buffer, showed a distinct drop off. Tests should be run, keeping the molarity of 0.9uM constant and varying other factors to determine the optimum molar ratio.
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Revision as of 22:48, 25 October 2012

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Objective

  • to run Fluorescence on Luminol-HRP assays.

Description

Please refer to table for volumes and concentrations of HRP, Luminol, Phenol, Buffer, and hydrogen peroxide used.

Image:20121024.png

Data

Image:Trail_6.png

  • All the trails, except trial 6 had a straight peak, showing do drop off of the fluorescence. Trail 6, containing, 454uL 0.564mM Luminol, 112.7uL 5mM hydrogen peroxide, 16.5uL 0.9uM HRP, 13.2 uL 18mM 4-Iodophenol, and 1000uL Carbonate Buffer, showed a distinct drop off. Tests should be run, keeping the molarity of 0.9uM constant and varying other factors to determine the optimum molar ratio.


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