User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/10/24 - Revision history

Dhea Patel: /* Description */

Dhea Patel — Fri, 07 Dec 2012 04:53:00 GMT

Description

←Older revision		Revision as of 04:53, 7 December 2012
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	\| align="center" style="background:#f0f0f0;"\|''''''		\| align="center" style="background:#f0f0f0;"\|''''''
	\|-		\|-
-	\| Trial\|\|Luminol\|\|H<sub>2</sub>O<sub>2</sub>\|\|4-Iodophenol\|\|HRP\|\|Water\|\|Carbonate Buffer	+	\| Trial\|\|Luminol\|\|H<sub>2</sub>O<sub>2</sub>\|\|4-Iodophenol\|\|HRP\|\|Water\|\|0.833mM Sodium Carbonate Buffer
	\|-		\|-
	\| 1\|\|454 µL at 0.564 mM\|\|112.7 µL at 5 mM\|\|13.2 µL at 18 mM\|\|33 µL at 9.6 µM\|\|1033 µL\|\|x		\| 1\|\|454 µL at 0.564 mM\|\|112.7 µL at 5 mM\|\|13.2 µL at 18 mM\|\|33 µL at 9.6 µM\|\|1033 µL\|\|x

Dhea Patel: /* Description */

Dhea Patel — Fri, 07 Dec 2012 04:47:55 GMT

Description

←Older revision		Revision as of 04:47, 7 December 2012
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	Please refer to table for volumes and concentrations of HRP, Luminol, Phenol, Buffer, and hydrogen peroxide used.		Please refer to table for volumes and concentrations of HRP, Luminol, Phenol, Buffer, and hydrogen peroxide used.

-	[~~[Image~~:~~20121024~~.~~png]]~~	+	{\| {{table}}
		+	\| align="center" style="background:#f0f0f0;"\|''''''
		+	\| align="center" style="background:#f0f0f0;"\|'''Volume and Stock Concentration Added to Cuvette'''
		+	\| align="center" style="background:#f0f0f0;"\|''''''
		+	\| align="center" style="background:#f0f0f0;"\|''''''
		+	\| align="center" style="background:#f0f0f0;"\|''''''
		+	\| align="center" style="background:#f0f0f0;"\|'''Volume Added to Cuvette [µL]'''
		+	\| align="center" style="background:#f0f0f0;"\|''''''
		+	\|-
		+	\| Trial\|\|Luminol\|\|H<sub>2</sub>O<sub>2</sub>\|\|4-Iodophenol\|\|HRP\|\|Water\|\|Carbonate Buffer
		+	\|-
		+	\| 1\|\|454 µL at 0.564 mM\|\|112.7 µL at 5 mM\|\|13.2 µL at 18 mM\|\|33 µL at 9.6 µM\|\|1033 µL\|\|x
		+	\|-
		+	\| 2\|\|454 µL at 0.564 mM\|\|112.7 µL at 5 mM\|\|13.2 µL at 18 mM\|\|33 µL at 9.6 µM\|\|1033 µL\|\|x
		+	\|-
		+	\| 3\|\|454 µL at 0.564 mM\|\|112.7 µL at 5 mM\|\|13.2 µL at 18 mM\|\|33 µL at 9.6 µM\|\|2000 µL\|\|x
		+	\|-
		+	\| 4\|\|454 µL at 0.564 mM\|\|112.7 µL at 5 mM\|\|13.2 µL at 18 mM\|\|66 µL at 0.96 µM\|\|2000 µL\|\|x
		+	\|-
		+	\| 5\|\|454 µL at 0.564 mM\|\|112.7 µL at 5 mM\|\|13.2 µL at 18 mM\|\|33 µL at 2.3 µM\|\|x\|\|1000 µL
		+	\|-
		+	\| 6\|\|454 µL at 0.564 mM\|\|112.7 µL at 5 mM\|\|13.2 µL at 18 mM\|\|16.5 µL at 2.3 µM\|\|x\|\|1000 µL
		+	\|-
		+	\| 7\|\|454 µL at 0.564 mM\|\|112.7 µL at 5 mM\|\|13.2 µL at 18 mM\|\|16.5 µL at 0.96 µM\|\|x\|\|1000 µL
		+	\|-
		+	\| 8\|\|454 µL at 0.564 mM\|\|112.7 µL at 5 mM\|\|13.2 µL at 18 mM\|\|16.5 µL at 2.3 µM\|\|x\|\|2000 µL
		+	\|}

	==Data==		==Data==

Dhea Patel: /* Objective */

Dhea Patel — Fri, 26 Oct 2012 02:48:43 GMT

Objective

←Older revision		Revision as of 02:48, 26 October 2012
Line 17:		Line 17:
	==Objective==		==Objective==

-	~~#To make biocompatible protein films with specific reactive sites~~	+	*to run Fluorescence on Luminol-HRP assays.
-	~~#Explore the solution conditions (pH, ionic strength, buffer, stabilizers) that produce stable protein – gold nanoparticle suspensions~~	+

		+	==Description==
		+
		+	Please refer to table for volumes and concentrations of HRP, Luminol, Phenol, Buffer, and hydrogen peroxide used.
		+
		+	[[Image:20121024.png]]
		+
		+	==Data==
		+
		+	[[Image:Trail_6.png]]
		+	*All the trails, except trial 6 had a straight peak, showing do drop off of the fluorescence. Trail 6, containing, 454uL 0.564mM Luminol, 112.7uL 5mM hydrogen peroxide, 16.5uL 0.9uM HRP, 13.2 uL 18mM 4-Iodophenol, and 1000uL Carbonate Buffer, showed a distinct drop off. Tests should be run, keeping the molarity of 0.9uM constant and varying other factors to determine the optimum molar ratio.

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Dhea Patel: Autocreate 2012/10/24 Entry for User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook

Dhea Patel — Wed, 24 Oct 2012 16:17:21 GMT

Autocreate 2012/10/24 Entry for User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook

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==Objective==

#To make biocompatible protein films with specific reactive sites
#Explore the solution conditions (pH, ionic strength, buffer, stabilizers) that produce stable protein – gold nanoparticle suspensions


|}

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