User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/11/04

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(Autocreate 2012/11/04 Entry for User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook)
Current revision (20:46, 4 November 2012) (view source)
 
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==Objective==
==Objective==
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#To make biocompatible protein films with specific reactive sites
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*to express ADA in LB media
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#Explore the solution conditions (pH, ionic strength, buffer, stabilizers)  that produce stable protein – gold nanoparticle suspensions
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*to extract cells from media
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==Description==
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Please refer to [[http://openwetware.org/wiki/User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/11/04| Kay's entry]] for details and protocol for:
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*centrifuging the cells,
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*preparing IPTG,
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*preparing ADA protein expression culture,
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*Inoculating the cells,
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* Centrifuging of the cells (again),
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*Resuspention of cell pellets in [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/09/26| binding buffer]],
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*and storing the resuspended cells.

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Objective

  • to express ADA in LB media
  • to extract cells from media

Description

Please refer to [Kay's entry] for details and protocol for:

  • centrifuging the cells,
  • preparing IPTG,
  • preparing ADA protein expression culture,
  • Inoculating the cells,
  • Centrifuging of the cells (again),
  • Resuspention of cell pellets in binding buffer,
  • and storing the resuspended cells.



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