User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/11/06

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(Objective)
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*to run FPLC on the ADA protein from [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/11/03| the third]] and [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/11/04 | the fourth]] of November.
*to run FPLC on the ADA protein from [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/11/03| the third]] and [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/11/04 | the fourth]] of November.
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==Description==
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*The cells from [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/11/04 | the fourth]] of November were sonciated for 30 seconds and then placed in an ice bath for 30 seconds. This was repeated for a total of 3 times.
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*The cells were then transferred to centrifuge tubes, balanced, and placed in the centrifuge at 18,000rpm at 4°C for 2 hours.
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*The cells were then filtered using...
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*The cells were then run on FPLC, using the binding buffer and elusion buffer from [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/09/26| September 26th]].
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*The cells were collected in 5 mL aliquots and transferred into a Falcon tube.
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*The [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/10/31|Au/Lysozyme solutions]] were obtained from the oven. The following images are the results from the Au/Lysozyme reaction.
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*[[Image:IMAG0851.jpg]]
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*[[Image:IMAG0852.jpg]]
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Revision as of 14:15, 6 November 2012

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Objective

  • to run transformations of PCR product into E. coli cells
  • to run FPLC on the ADA protein from the third and the fourth of November.

Description

  • The cells from the fourth of November were sonciated for 30 seconds and then placed in an ice bath for 30 seconds. This was repeated for a total of 3 times.
  • The cells were then transferred to centrifuge tubes, balanced, and placed in the centrifuge at 18,000rpm at 4°C for 2 hours.
  • The cells were then filtered using...
  • The cells were then run on FPLC, using the binding buffer and elusion buffer from September 26th.
  • The cells were collected in 5 mL aliquots and transferred into a Falcon tube.


  • The Au/Lysozyme solutions were obtained from the oven. The following images are the results from the Au/Lysozyme reaction.
  • Image:IMAG0851.jpg
  • Image:IMAG0852.jpg


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