User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2013/01/23

From OpenWetWare

Jump to: navigation, search
Current revision (16:24, 23 January 2013) (view source)
 
(7 intermediate revisions not shown.)
Line 20: Line 20:
*To complete experimental protocol for next week.
*To complete experimental protocol for next week.
-
==Project Proposal==
+
 
==Experimental Protocol==
==Experimental Protocol==
-
'''Preparing Phosphate Buffer'''
+
'''Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)'''
 +
 
 +
*Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L.
 +
**The pH of the final solution will be 7.4.
 +
**This buffer can be stored for up to 1 mo at 4°C.
 +
*Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)
'''Running ADA Kinetics'''
'''Running ADA Kinetics'''
-
*The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed on [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2013/01/29]].
+
*The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2013/01/29| next Tuesday]].
 +
 
 +
[[Image:ADA_kinetics_table.png|px80]]
 +
 
 +
*To prepare a 30 mM stock solution of adenosine:
 +
 
 +
<math>\frac{0.030mol}{L}</math> of adenosine × <math>\frac{267.24  g}{1  mol}</math> = <math>\frac{8.0172g}{L}</math> of adenosine in buffer
 +
 
 +
*To make 10mL of solution, 0.080172g of adenosine will be added to 10mL of phosphate buffer.
 +
-
[[Image:ADA_kinetics_table.png]]
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Current revision

Search this Project

Customize your entry pages

Objective

  • To complete project proposal
  • To complete experimental protocol for next week.


Experimental Protocol

Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)

  • Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L.
    • The pH of the final solution will be 7.4.
    • This buffer can be stored for up to 1 mo at 4°C.
  • Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)

Running ADA Kinetics

  • The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed next Tuesday.

px80

  • To prepare a 30 mM stock solution of adenosine:

\frac{0.030mol}{L} of adenosine × \frac{267.24  g}{1  mol} = \frac{8.0172g}{L} of adenosine in buffer

  • To make 10mL of solution, 0.080172g of adenosine will be added to 10mL of phosphate buffer.



Personal tools