User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2013/01/23

Current revision

Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)

Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L.
- The pH of the final solution will be 7.4.
- This buffer can be stored for up to 1 mo at 4°C.
Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)

Running ADA Kinetics

The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed next Tuesday.

$\frac{0.030mol}{L}$ of adenosine × $\frac{267.24 g}{1 mol}$ = $\frac{8.0172g}{L}$ of adenosine in buffer

To make 10mL of solution, 0.080172g of adenosine will be added to 10mL of phosphate buffer.

@@ Line 20: / Line 20: @@
 *To complete experimental protocol for next week.
-==Project Proposal==
 ==Experimental Protocol==
-'''Preparing Phosphate Buffer'''
+'''Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)'''
+*Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L.
+**The pH of the final solution will be 7.4.
+**This buffer can be stored for up to 1 mo at 4°C.
+*Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)
 '''Running ADA Kinetics'''
@@ Line 31: / Line 36: @@
 [[Image:ADA_kinetics_table.png|px80]]
+*To prepare a 30 mM stock solution of adenosine:
+<math>\frac{0.030mol}{L}</math> of adenosine × <math>\frac{267.24  g}{1  mol}</math> = <math>\frac{8.0172g}{L}</math> of adenosine in buffer
+*To make 10mL of solution, 0.080172g of adenosine will be added to 10mL of phosphate buffer.
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