User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2013/01/23

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 Revision as of 16:15, 23 January 2013 (view source) (→Experimental Protocol)← Previous diff Current revision (16:24, 23 January 2013) (view source) (4 intermediate revisions not shown.) Line 20: Line 20: *To complete experimental protocol for next week. *To complete experimental protocol for next week. - ==Project Proposal== + ==Experimental Protocol== ==Experimental Protocol== Line 36: Line 36: [[Image:ADA_kinetics_table.png|px80]] [[Image:ADA_kinetics_table.png|px80]] + + *To prepare a 30 mM stock solution of adenosine: + + $\frac{0.030mol}{L}$ of adenosine × $\frac{267.24 g}{1 mol}$ = $\frac{8.0172g}{L}$ of adenosine in buffer + + *To make 10mL of solution, 0.080172g of adenosine will be added to 10mL of phosphate buffer. + +

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Objective

• To complete project proposal
• To complete experimental protocol for next week.

Experimental Protocol

Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)

• Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L.
• The pH of the final solution will be 7.4.
• This buffer can be stored for up to 1 mo at 4°C.
• Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)

• The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed next Tuesday.

• To prepare a 30 mM stock solution of adenosine:

$\frac{0.030mol}{L}$ of adenosine × $\frac{267.24 g}{1 mol}$ = $\frac{8.0172g}{L}$ of adenosine in buffer

• To make 10mL of solution, 0.080172g of adenosine will be added to 10mL of phosphate buffer.