User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2013/01/23

Current revision

Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)

Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L.
- The pH of the final solution will be 7.4.
- This buffer can be stored for up to 1 mo at 4°C.
Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)

Running ADA Kinetics

The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed next Tuesday.

$\frac{0.030mol}{L}$ of adenosine × $\frac{267.24 g}{1 mol}$ = $\frac{8.0172g}{L}$ of adenosine in buffer

To make 10mL of solution, 0.080172g of adenosine will be added to 10mL of phosphate buffer.

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 *To prepare a 30 mM stock solution of adenosine:
-<math>\frac{0.030mol}{L}</math> of adenosine × <math>\frac{267.24  g}{1  mol}</math> = <math>\frac{8.0172g adenosine}{L buffer}
+<math>\frac{0.030mol}{L}</math> of adenosine × <math>\frac{267.24  g}{1  mol}</math> = <math>\frac{8.0172g}{L}</math> of adenosine in buffer
+*To make 10mL of solution, 0.080172g of adenosine will be added to 10mL of phosphate buffer.