User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2013/01/23

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(Experimental Protocol)
(Experimental Protocol)
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==Experimental Protocol==
==Experimental Protocol==
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'''Preparing Phosphate Buffer'''
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'''Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)'''
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*Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L.
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**The pH of the final solution will be 7.4.
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**This buffer can be stored for up to 1 mo at 4°C.
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*Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)
'''Running ADA Kinetics'''
'''Running ADA Kinetics'''

Revision as of 15:15, 23 January 2013

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Objective

  • To complete project proposal
  • To complete experimental protocol for next week.

Project Proposal

Experimental Protocol

Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)

  • Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L.
    • The pH of the final solution will be 7.4.
    • This buffer can be stored for up to 1 mo at 4°C.
  • Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)

Running ADA Kinetics

  • The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed next Tuesday.

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