User:Dhea Patel/Notebook/Hemoglobin Project/2013/01/25

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*each solvent was used as a blank.
*each solvent was used as a blank.
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*The data was corrected for the blank and a corrected baseline.  
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*The data was corrected for the blank and a corrected baseline.
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*Fluorescence was run with an emission scan of 310-500nm and excitation at 290nm. The emission and excitation slits were 10.0nm, and the scan speed (nm/min) was 100.
==Data==
==Data==

Revision as of 09:53, 1 February 2013

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Objective

  • To run UV-vis and Fluorescence on 0.0058g Myoglobin + 0.4961g KCl in 20mL 0.01M pH 7 phosphate buffer (evaporated) in various organic solvents.

Description

1. 10.6mg of the solid (0.0058g Myoglobin + 0.4961g KCl in 20mL 0.01M pH 7 phosphate buffer (evaporated)) was added to 1 mL of MeOH. 2. 11.0mg of the solid was added to 1mL of Acetone 3. 11.1mg of the solid was added to 1mL Acetonitrile 4. 11.3mg of the solid was added to 1mL Ethyl Acetate 5. 11.3mg of the solid was added to 1mL water 6. 11.4mg of the solid was added to 1mL chloroform

  • each solvent was used as a blank.
  • The data was corrected for the blank and a corrected baseline.
  • Fluorescence was run with an emission scan of 310-500nm and excitation at 290nm. The emission and excitation slits were 10.0nm, and the scan speed (nm/min) was 100.

Data

  • UV-vis of solid in MeOH

px10

  • Fluorescence of solid in MeOH

px10

  • UV-vis of solid in Acetone

px10

  • Fluorescence of solid in Acetone

px10

  • UV-vis of solid in Acetonitrile

px10

  • Fluorescence of solid in Acetonitrile

px10

  • UV-vis of solid in Ethyl Acetate

px10

  • Fluorescence of solid in Ethyl Acetate

px10

  • UV-vis of solid in Water

px10

  • Fluorescence of solid in Water

px10

  • UV-vis of solid in Chloroform

px10

  • Fluorescence of solid in Chloroform

px10


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