User:Dhea Patel/Notebook/Hemoglobin Project/2013/01/25: Difference between revisions
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==Description== | ==Description== | ||
# 10.6mg of the solid (0.0058g Myoglobin + 0.4961g KCl in 20mL 0.01M pH 7 phosphate buffer (evaporated)) was added to 1 mL of MeOH. | |||
# 11.0mg of the solid was added to 1mL of Acetone | |||
# 11.1mg of the solid was added to 1mL Acetonitrile | |||
# 11.3mg of the solid was added to 1mL Ethyl Acetate | |||
# 11.3mg of the solid was added to 1mL water | |||
# 11.4mg of the solid was added to 1mL chloroform | |||
*each solvent was used as a blank. | *each solvent was used as a blank. | ||
*The data was corrected for the blank and a corrected baseline. | *The data was corrected for the blank and a corrected baseline. | ||
*UV-vis scanned from 200-800nm | |||
*Fluorescence was run with an emission scan of 310-500nm and excitation at 290nm. The emission and excitation slits were 10.0nm, and the scan speed (nm/min) was 100. | *Fluorescence was run with an emission scan of 310-500nm and excitation at 290nm. The emission and excitation slits were 10.0nm, and the scan speed (nm/min) was 100. | ||
Revision as of 08:22, 1 February 2013
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