User:Dhea Patel/Notebook/Hemoglobin Project/2013/01/25
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| - | == | + | ==Objective== |
| - | * | + | *To run UV-vis and Fluorescence on 0.0058g Myoglobin + 0.4961g KCl in 20mL 0.01M pH 7 phosphate buffer (evaporated) in various organic solvents. |
| + | ==Description== | ||
| + | # 10.6mg of the solid (0.0058g Myoglobin + 0.4961g KCl in 20mL 0.01M pH 7 phosphate buffer (evaporated)) was added to 1 mL of MeOH. | ||
| + | # 11.0mg of the solid was added to 1mL of Acetone | ||
| + | # 11.1mg of the solid was added to 1mL Acetonitrile | ||
| + | # 11.3mg of the solid was added to 1mL Ethyl Acetate | ||
| + | # 11.3mg of the solid was added to 1mL water | ||
| + | # 11.4mg of the solid was added to 1mL chloroform | ||
| + | |||
| + | *each solvent was used as a blank. | ||
| + | *The data was corrected for the blank and a corrected baseline. | ||
| + | |||
| + | *UV-vis scanned from 200-800nm | ||
| + | *Fluorescence was run with an emission scan of 310-500nm and excitation at 290nm. The emission and excitation slits were 10.0nm, and the scan speed (nm/min) was 100. | ||
| + | |||
| + | ==Data== | ||
| + | |||
| + | *UV-vis of solid in MeOH | ||
| + | [[Image:UV-vis_of_10.6mg_solid_in_1mL_of_MeOH.png|px10]] | ||
| + | *Fluorescence of solid in MeOH | ||
| + | [[Image:Fluorescence_of_10.6mg_solid_in_1mL_MeOH.png|px10]] | ||
| + | |||
| + | *UV-vis of solid in Acetone | ||
| + | [[Image:UV-vis_of_solid_in_Acetone.png|px10]] | ||
| + | *Fluorescence of solid in Acetone | ||
| + | [[Image:Fluorescence_of_11.0mg_solid_in_1mL_Acetone.png|px10]] | ||
| + | |||
| + | *UV-vis of solid in Acetonitrile | ||
| + | [[Image:UV-vis_of_11.0mg_of_solid_in_1mL_of_Acetonitrile.png|px10]] | ||
| + | *Fluorescence of solid in Acetonitrile | ||
| + | [[Image:Fluorescence_of_11.3mg_solid_in_1mL_Acetonitrile.png|px10]] | ||
| + | |||
| + | *UV-vis of solid in Ethyl Acetate | ||
| + | [[Image:UV-vis_of_solid_in_Ethyl_Acetate.png|px10]] | ||
| + | *Fluorescence of solid in Ethyl Acetate | ||
| + | [[Image:Fluorescence_of_11.3mg_solid_in_1mL_Ethyl_Acetate.png|px10]] | ||
| + | |||
| + | *UV-vis of solid in Water | ||
| + | [[Image:UV-vis of 11.3mg of solid in 1mL of water.png|px10]] | ||
| + | *Fluorescence of solid in Water | ||
| + | [[Image:Fluorescence_of_11.3mg_solid_in_1mL_Water.png|px10]] | ||
| + | |||
| + | *UV-vis of solid in Chloroform | ||
| + | [[Image:UV-vis_of_solid_in_Chloroform.png|px10]] | ||
| + | *Fluorescence of solid in Chloroform | ||
| + | [[Image:Fluorescence_of_11.4mg_solid_in_1mL_Chloroform.png|px10]] | ||
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