User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/01: Difference between revisions
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== | ==Objective== | ||
* | *To run UV-vis and Fluorescence on 0.0050g Myoglobin + 0.4856g KCl in 20mL 0.01M pH 5 acetate buffer (evaporated) in various organic solvents. | ||
==Description== | |||
# 9.3mg of the solid (0.0050g Myoglobin + 0.4856g KCl in 20mL 0.01M pH 5 acetate buffer (evaporated)) was added to 1 mL of MeOH. | |||
# 9.3mg of the solid was added to 1mL of Acetone | |||
# 10.4mg of the solid was added to 1mL Acetonitrile | |||
# 9.9mg of the solid was added to 1mL Ethyl Acetate | |||
# 10.0mg of the solid was measured | |||
# 10.2mg of the solid was measured | |||
*all the samples were vortexed and centrifuged before being measured | |||
* Centrifuge settings were as follows: | |||
**13200rpm | |||
**0:16 seconds | |||
**851 rotor | |||
*each solvent was used as a blank. | |||
*The data was corrected for the blank and a corrected baseline. | |||
*UV-vis scanned from 200-800nm | |||
*Fluorescence was run with an emission scan of 310-500nm and excitation at 290nm. The emission and excitation slits were 10.0nm, and the scan speed (nm/min) was 100. | |||
==Data== | |||
*UV-vis of solid in MeOH | |||
[[Image:9.3mg_Acetate_Solid_in_1mL_MeOH.png]] | |||
*Fluorescence of solid in MeOH | |||
[[Image:Fluorescence_of_9.3mg_Citrate_Solid_in_1mL_MeOH.png]] | |||
*UV-vis of solid in Acetone | |||
[[Image:9.3mg_Acetate_Solid_in_1mL_Acetone_.png]] | |||
*Fluorescence of solid in Acetone | |||
[[Image:Fluorescence_of_9.3mg_Citrate_Solid_in_1mL_Acetone.png]] | |||
*UV-vis of solid in Acetonitrile | |||
[[Image:10.4mg_Acetate_Solid_in_1mL_Acetonitrile_.png]] | |||
*Fluorescence of solid in Acetonitrile | |||
[[Image:Fluorescence_of_10.4mg_Citrate_Solid_in_1mL_Acetonitrile.png]] | |||
*UV-vis of solid in Ethyl Acetate | |||
[[Image:9.9_Acetate_Solid_in_1mL_Ethyl_Acetate.png]] | |||
*Fluorescence of solid in Ethyl Acetate | |||
[[Image:Fluorescence_of_9.9mg_Citrate_Solid_in_1mL_Ethyl_Acetate.png]] | |||
*UV-vis of solid in Water | |||
[[Image:10mg_Acetate_Solid_in_Water.png]] | |||
*Fluorescence of solid in Water | |||
[[Image:Fluorescence_of_10.0mg_Acetate_Solid_in_1mL_Water.png]] | |||
*UV-vis of solid in Chloroform | |||
[[Image:10.2mg_Acetate_in_Chloroform.png]] | |||
*Fluorescence of solid in Chloroform | |||
[[Image:Fluorescence_of_10.2mg_Acetate_Solid_in_1mL_Chloroform.png]] | |||
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Revision as of 08:24, 19 February 2013
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Objective
Description
Data
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