Objective
- To run UV-vis and Fluorescence on 0.0050g Myoglobin + 0.4856g KCl in 20mL 0.01M pH 5 acetate buffer (evaporated) in various organic solvents.
Description
- 9.3mg of the solid (0.0050g Myoglobin + 0.4856g KCl in 20mL 0.01M pH 5 acetate buffer (evaporated)) was added to 1 mL of MeOH.
- 9.3mg of the solid was added to 1mL of Acetone
- 10.4mg of the solid was added to 1mL Acetonitrile
- 9.9mg of the solid was added to 1mL Ethyl Acetate
- 10.0mg of the solid was measured
- 10.2mg of the solid was measured
- all the samples were vortexed and centrifuged before being measured
- Centrifuge settings were as follows:
- 13200rpm
- 0:16 seconds
- 851 rotor
- each solvent was used as a blank.
- The data was corrected for the blank and a corrected baseline.
- UV-vis scanned from 200-800nm
- Fluorescence was run with an emission scan of 310-500nm and excitation at 290nm. The emission and excitation slits were 10.0nm, and the scan speed (nm/min) was 100.
Data
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- Fluorescence of solid in MeOH
- UV-vis of solid in Acetone
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- Fluorescence of solid in Acetone
- UV-vis of solid in Acetonitrile
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- Fluorescence of solid in Acetonitrile
- UV-vis of solid in Ethyl Acetate
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- Fluorescence of solid in Ethyl Acetate
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- Fluorescence of solid in Water
- UV-vis of solid in Chloroform
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- Fluorescence of solid in Chloroform
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Project name
