Revision as of 12:02, 20 February 2013

Project name

WP:TOC

Objective

to finish running samples from Friday
to run samples of solid (0.2g p-sorbitol , 0.01 Mb, 0.1g PEG in 10mL citrate buffer) made January 29th.

10.0mg of the "Myoglobin in Citrate buffer" solid was added to 1mL water
10.2mg of the "Myoglobin in Citrate buffer" solid was added to 1mL chloroform
10.0mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL water
11.4mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL chloroform
1. the solid remained in a clump and floated to the top of the tube.
9.8mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL ethyl acetate
9.5mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL acetonitrile
11.9mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL acetone
9.7mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL methanol

all the samples were vortexed and centrifuged before being measured
Centrifuge settings were as follows:
- 13200rpm
- 0:16 seconds
- 851 rotor
each solvent was used as a blank.
The data was corrected for the blank and a corrected baseline.
UV-vis scanned from 200-800nm
Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min.

Please see last Friday's Data for the UV-vis and Fluorescence spectra of the myoglobin samples.

[[]]

[[]]

@@ Line 57: / Line 57: @@
 [[Image:9.8mg_psorbitol_CitrateB_Solid_in_1mL_Ethyl_Acetate_.png]]
 *Fluorescence of "p-sorbitol in Citrate buffer" solid in Ethyl Acetate
-[[]]
+[[Image:Fluorescence_of_9.8mg_p-sorbitol_in_Citrate_Buffer_Solid_in_1mL_Ethyl_Acetate_.png]]
 *UV-vis of "p-sorbitol in Citrate buffer" solid in Water
 [[Image:10.0mg_psorbitol_CitrateB_Solid_in_1mL_Water.png]]
 *Fluorescence of "p-sorbitol in Citrate buffer" solid in Water
-[[]]
+[[Image:Fluorescence_of_10.0mg_p-sorbitol_in_Citrate_Buffer_Solid_in_1mL_Water_.png]]
 *UV-vis of "p-sorbitol in Citrate buffer" solid in Chloroform