User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/05

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

WP:TOC

Objective

  • to finish running samples from Friday
  • to run samples of solid (0.2g p-sorbitol , 0.01 Mb, 0.1g PEG in 10mL citrate buffer) made January 29th.

Description

  1. 10.0mg of the "Myoglobin in Citrate buffer" solid was added to 1mL water
  2. 10.2mg of the "Myoglobin in Citrate buffer" solid was added to 1mL chloroform
  3. 10.0mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL water
  4. 11.4mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL chloroform
    1. the solid remained in a clump and floated to the top of the tube.
  5. 9.8mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL ethyl acetate
  6. 9.5mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL acetonitrile
  7. 11.9mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL acetone
  8. 9.7mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL methanol
  • all the samples were vortexed and centrifuged before being measured
  • Centrifuge settings were as follows:
    • 13200rpm
    • 0:16 seconds
    • 851 rotor
  • each solvent was used as a blank.
  • The data was corrected for the blank and a corrected baseline.
  • UV-vis scanned from 200-800nm
  • Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min.

Data

  • Please see last Friday's Data for the UV-vis and Fluorescence spectra of the myoglobin samples.


  • UV-vis of "p-sorbitol in Citrate buffer" solid in MeOH

Image:9.7mg_psorbitol_citrate_in_MeOH.png

  • Fluorescence of "p-sorbitol in Citrate buffer" solid in MeOH

[[]]

  • UV-vis of "p-sorbitol in Citrate buffer" solid in Acetone

Image:11.9mg_psorbitol_CitrateB_Solid_in_1mL_Acetone.png

  • Fluorescence of "p-sorbitol in Citrate buffer" solid in Acetone

[[]]

  • UV-vis of "p-sorbitol in Citrate buffer" solid in Acetonitrile

Image:9.5mg_psorbitol_CitrateB_Solid_in_1mL_Acetonitrile_.png

  • Fluorescence of "p-sorbitol in Citrate buffer" solid in Acetonitrile

[[]]

  • UV-vis of "p-sorbitol in Citrate buffer" solid in Ethyl Acetate

Image:9.8mg_psorbitol_CitrateB_Solid_in_1mL_Ethyl_Acetate_.png

  • Fluorescence of "p-sorbitol in Citrate buffer" solid in Ethyl Acetate

[[]]

  • UV-vis of "p-sorbitol in Citrate buffer" solid in Water

Image:10.0mg_psorbitol_CitrateB_Solid_in_1mL_Water.png

  • Fluorescence of "p-sorbitol in Citrate buffer" solid in Water

[[]]

  • UV-vis of "p-sorbitol in Citrate buffer" solid in Chloroform

Image:11.4mg_psorbitol_CitrateB_Solid_in_1mL_Chloroform.png

  • Fluorescence of "p-sorbitol in Citrate buffer" solid in Chloroform

Image:Fluorescence_of_11.4mg_p-sorbitol_CitrateB_Solid_in_1mL_Chloroform.png


Personal tools