User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/06 - Revision history

Dhea Patel at 16:13, 19 February 2013

Dhea Patel — Tue, 19 Feb 2013 16:13:21 GMT

←Older revision		Revision as of 16:13, 19 February 2013
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	*UV-vis of "p-sorbitol in Acetate buffer" solid in Acetone		*UV-vis of "p-sorbitol in Acetate buffer" solid in Acetone
-	[[Image:11.~~9mg_psorbitol_AcetateB_Solid_in_1mL_Acetone~~.png]]	+	[[Image:9.3mg_psorbitol_AcetateB_Solid_in_1mL_Acetone.png]]
	*Fluorescence of "p-sorbitol in Acetate buffer" solid in Acetone		*Fluorescence of "p-sorbitol in Acetate buffer" solid in Acetone
	[[]]		[[]]

Dhea Patel: /* Data */

Dhea Patel — Tue, 19 Feb 2013 16:09:58 GMT

Data

Dhea Patel: /* Description */

Dhea Patel — Wed, 06 Feb 2013 17:11:39 GMT

Description

←Older revision		Revision as of 17:11, 6 February 2013
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	# 9.8mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL water, centrifuged, and measured		# 9.8mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL water, centrifuged, and measured
	# 9.5mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL chloroform, centrifuged, and measured		# 9.5mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL chloroform, centrifuged, and measured
		+	##the solid remained in a clump and floated to the top of the tube.
	# 11.9mg of the "p-sorbitol in Citrate buffer" solid in 1mL acetone was centrifuged, and measured		# 11.9mg of the "p-sorbitol in Citrate buffer" solid in 1mL acetone was centrifuged, and measured
		+	##as this mixture sat in the fridge over night, the solid began to dissipate and float to the top.
	# 9.7mg of the "p-sorbitol in Citrate buffer" solid in 1mL methanol was centrifuged, and measured		# 9.7mg of the "p-sorbitol in Citrate buffer" solid in 1mL methanol was centrifuged, and measured

Dhea Patel at 15:27, 6 February 2013

Dhea Patel — Wed, 06 Feb 2013 15:27:13 GMT

←Older revision		Revision as of 15:27, 6 February 2013
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	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==Objective==
-	* ~~Insert content here~~...	+	*to finish running samples from [[User:Dhea_Patel/Notebook/Hemoglobin_Project/2013/02/01\|Friday]]
		+	*to run samples of solid (0.2g p-sorbitol , 0.01 Mb, 0.1g PEG in 10mL citrate buffer) made January 29th.

		+	==Description==
		+
		+	# 10.0mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL methanol, centrifuged, and measured
		+	# 10.2mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL acetone, centrifuged, and measured
		+	# 10.0mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL acetonitrile, centrifuged, and measured
		+	# 11.4mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL ethyl acetate, centrifuged, and measured
		+	# 9.8mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL water, centrifuged, and measured
		+	# 9.5mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL chloroform, centrifuged, and measured
		+	# 11.9mg of the "p-sorbitol in Citrate buffer" solid in 1mL acetone was centrifuged, and measured
		+	# 9.7mg of the "p-sorbitol in Citrate buffer" solid in 1mL methanol was centrifuged, and measured
		+
		+	*all the samples were vortexed and centrifuged before being measured
		+	* Centrifuge settings were as follows:
		+	**13200rpm
		+	**0:16 seconds
		+	**851 rotor
		+	* Each solvent was used as a blank.
		+	* The data was corrected for the blank and a corrected baseline.
		+	* UV-vis scanned from 200-800nm
		+	* Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min.
		+
		+	=Data=
		+
		+	*Please see [[User:Dhea_Patel/Notebook/Hemoglobin_Project/2013/02/05#Data\|yesterday's Data]] for the UV-vis and Fluorescence spectra of the p-sorbitol in Citrate samples.
		+
		+
		+	*UV-vis of "p-sorbitol in Acetate buffer" solid in MeOH
		+	[[]]
		+	*Fluorescence of "p-sorbitol in Acetate buffer" solid in MeOH
		+	[[]]
		+
		+	*UV-vis of "p-sorbitol in Acetate buffer" solid in Acetone
		+	[[]]
		+	*Fluorescence of "p-sorbitol in Acetate buffer" solid in Acetone
		+	[[]]
		+
		+	*UV-vis of "p-sorbitol in Acetate buffer" solid in Acetonitrile
		+	[[]]
		+	*Fluorescence of "p-sorbitol in Acetate buffer" solid in Acetonitrile
		+	[[]]
		+
		+	*UV-vis of "p-sorbitol in Acetate buffer" solid in Ethyl Acetate
		+	[[]]
		+	*Fluorescence of "p-sorbitol in Acetate buffer" solid in Ethyl Acetate
		+	[[]]
		+
		+	*UV-vis of "p-sorbitol in Acetate buffer" solid in Water
		+	[[]]
		+	*Fluorescence of "p-sorbitol in Acetate buffer" solid in Water
		+	[[]]
		+
		+	*UV-vis of "p-sorbitol in Acetate buffer" solid in Chloroform
		+	[[]]
		+	*Fluorescence of "p-sorbitol in Acetate buffer" solid in Chloroform
		+	[[]]

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Dhea Patel: Autocreate 2013/02/06 Entry for User:Dhea_Patel/Notebook/Hemoglobin_Project

Dhea Patel — Wed, 06 Feb 2013 15:00:46 GMT

Autocreate 2013/02/06 Entry for User:Dhea_Patel/Notebook/Hemoglobin_Project

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

__NOTOC__

←Older revision		Revision as of 16:09, 19 February 2013
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	*UV-vis of "p-sorbitol in Acetate buffer" solid in MeOH		*UV-vis of "p-sorbitol in Acetate buffer" solid in MeOH
-	[[]]	+	[[Image:12.5mg_psorbitol_acetate_in_MeOH.png]]
	*Fluorescence of "p-sorbitol in Acetate buffer" solid in MeOH		*Fluorescence of "p-sorbitol in Acetate buffer" solid in MeOH
	[[]]		[[]]

	*UV-vis of "p-sorbitol in Acetate buffer" solid in Acetone		*UV-vis of "p-sorbitol in Acetate buffer" solid in Acetone
-	[[]]	+	[[Image:11.9mg_psorbitol_AcetateB_Solid_in_1mL_Acetone.png]]
	*Fluorescence of "p-sorbitol in Acetate buffer" solid in Acetone		*Fluorescence of "p-sorbitol in Acetate buffer" solid in Acetone
	[[]]		[[]]

	*UV-vis of "p-sorbitol in Acetate buffer" solid in Acetonitrile		*UV-vis of "p-sorbitol in Acetate buffer" solid in Acetonitrile
-	[[]]	+	[[Image:10.7mg_psorbitol_AcetateB_Solid_in_1mL_Acetonitrile_.png]]
	*Fluorescence of "p-sorbitol in Acetate buffer" solid in Acetonitrile		*Fluorescence of "p-sorbitol in Acetate buffer" solid in Acetonitrile
	[[]]		[[]]

	*UV-vis of "p-sorbitol in Acetate buffer" solid in Ethyl Acetate		*UV-vis of "p-sorbitol in Acetate buffer" solid in Ethyl Acetate
-	[[]]	+	[[Image:8.4mg_psorbitol_AcetateB_Solid_in_1mL_Ethyl_Acetate.png]]
	*Fluorescence of "p-sorbitol in Acetate buffer" solid in Ethyl Acetate		*Fluorescence of "p-sorbitol in Acetate buffer" solid in Ethyl Acetate
	[[]]		[[]]

	*UV-vis of "p-sorbitol in Acetate buffer" solid in Water		*UV-vis of "p-sorbitol in Acetate buffer" solid in Water
-	[[]]	+	[[Image:9.4mg_psorbitol_AcetateB_Solid_in_1mL_Water_.png]]
	*Fluorescence of "p-sorbitol in Acetate buffer" solid in Water		*Fluorescence of "p-sorbitol in Acetate buffer" solid in Water
	[[]]		[[]]

	*UV-vis of "p-sorbitol in Acetate buffer" solid in Chloroform		*UV-vis of "p-sorbitol in Acetate buffer" solid in Chloroform
-	[[]]	+	[[Image:8.9mg_psorbitol_AcetateB_Solid_in_1mL_Chloroform_.png]]
	*Fluorescence of "p-sorbitol in Acetate buffer" solid in Chloroform		*Fluorescence of "p-sorbitol in Acetate buffer" solid in Chloroform
	[[]]		[[]]