User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/06

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Objective

  • to finish running samples from Friday
  • to run samples of solid (0.2g p-sorbitol , 0.01 Mb, 0.1g PEG in 10mL citrate buffer) made January 29th.

Description

  1. 10.0mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL methanol, centrifuged, and measured
  2. 10.2mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL acetone, centrifuged, and measured
  3. 10.0mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL acetonitrile, centrifuged, and measured
  4. 11.4mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL ethyl acetate, centrifuged, and measured
  5. 9.8mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL water, centrifuged, and measured
  6. 9.5mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL chloroform, centrifuged, and measured
    1. the solid remained in a clump and floated to the top of the tube.
  7. 11.9mg of the "p-sorbitol in Citrate buffer" solid in 1mL acetone was centrifuged, and measured
    1. as this mixture sat in the fridge over night, the solid began to dissipate and float to the top.
  8. 9.7mg of the "p-sorbitol in Citrate buffer" solid in 1mL methanol was centrifuged, and measured
  • all the samples were vortexed and centrifuged before being measured
  • Centrifuge settings were as follows:
    • 13200rpm
    • 0:16 seconds
    • 851 rotor
  • Each solvent was used as a blank.
  • The data was corrected for the blank and a corrected baseline.
  • UV-vis scanned from 200-800nm
  • Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min.

Data

  • Please see yesterday's Data for the UV-vis and Fluorescence spectra of the p-sorbitol in Citrate samples.


  • UV-vis of "p-sorbitol in Acetate buffer" solid in MeOH

Image:12.5mg_psorbitol_acetate_in_MeOH.png

  • Fluorescence of "p-sorbitol in Acetate buffer" solid in MeOH

[[]]

  • UV-vis of "p-sorbitol in Acetate buffer" solid in Acetone

Image:9.3mg_psorbitol_AcetateB_Solid_in_1mL_Acetone.png

  • Fluorescence of "p-sorbitol in Acetate buffer" solid in Acetone

[[]]

  • UV-vis of "p-sorbitol in Acetate buffer" solid in Acetonitrile

Image:10.7mg_psorbitol_AcetateB_Solid_in_1mL_Acetonitrile_.png

  • Fluorescence of "p-sorbitol in Acetate buffer" solid in Acetonitrile

[[]]

  • UV-vis of "p-sorbitol in Acetate buffer" solid in Ethyl Acetate

Image:8.4mg_psorbitol_AcetateB_Solid_in_1mL_Ethyl_Acetate.png

  • Fluorescence of "p-sorbitol in Acetate buffer" solid in Ethyl Acetate

[[]]

  • UV-vis of "p-sorbitol in Acetate buffer" solid in Water

Image:9.4mg_psorbitol_AcetateB_Solid_in_1mL_Water_.png

  • Fluorescence of "p-sorbitol in Acetate buffer" solid in Water

[[]]

  • UV-vis of "p-sorbitol in Acetate buffer" solid in Chloroform

Image:8.9mg_psorbitol_AcetateB_Solid_in_1mL_Chloroform_.png

  • Fluorescence of "p-sorbitol in Acetate buffer" solid in Chloroform

[[]]


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