User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/12

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(Autocreate 2013/02/12 Entry for User:Dhea_Patel/Notebook/Hemoglobin_Project)
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==Entry title==
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==Objective==
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* Insert content here...
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* Run UV-vis and Fluorescence on p-sorbitol in phosphate buffer and mannitol in tris buffer samples in either MeOH, acetone, acetonitrile, ethyl acetate, water, or chloroform.
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==Description==
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*all the mannitol in tris buffer samples were vortexed and centrifuged before being measured
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*Centrifuge settings were as follows:
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**13200rpm
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**0:16 seconds
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**851 rotor
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*all the p-sorbitol in phosphate buffer samples were sonicated for 3 hours in their respective solvents.
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*Each solvent was used as a blank.
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*The data was corrected for the blank and a corrected baseline.
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*UV-vis scanned from 200-800nm
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*Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min.
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'''Only MeOH and Acetone samples (for both mannitol in tris and p-sorbitol in phosphate) were run today. The rest of the samples will be run [[User:Dhea_Patel/Notebook/Hemoglobin_Project/2013/02/13|tomorrow]].'''
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Revision as of 09:20, 13 February 2013

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Objective

  • Run UV-vis and Fluorescence on p-sorbitol in phosphate buffer and mannitol in tris buffer samples in either MeOH, acetone, acetonitrile, ethyl acetate, water, or chloroform.

Description

  • all the mannitol in tris buffer samples were vortexed and centrifuged before being measured
  • Centrifuge settings were as follows:
    • 13200rpm
    • 0:16 seconds
    • 851 rotor
  • all the p-sorbitol in phosphate buffer samples were sonicated for 3 hours in their respective solvents.
  • Each solvent was used as a blank.
  • The data was corrected for the blank and a corrected baseline.
  • UV-vis scanned from 200-800nm
  • Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min.

Only MeOH and Acetone samples (for both mannitol in tris and p-sorbitol in phosphate) were run today. The rest of the samples will be run tomorrow.


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