User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/12

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Objective Run UV-vis and Fluorescence on p-sorbitol in phosphate buffer and mannitol in tris buffer samples in either MeOH, acetone, acetonitrile, ethyl acetate, water, or chloroform. Description 11.7mg mannitol in Tris buffer, solvent: MeOH 10.0mg mannitol in Tris buffer, solvent: Acetone 9.4mg p-sorbitol in phosphate buffer, solvent: MeOH 10.1mg p-sorbitol in phosphate buffer, solvent: Acetone all the mannitol in tris buffer samples were vortexed and centrifuged before being measured Centrifuge settings were as follows: 13200rpm 0:16 seconds 851 rotor all the p-sorbitol in phosphate buffer samples were sonicated for 3 hours in their respective solvents. Each solvent was used as a blank. The data was corrected for the blank and a corrected baseline. UV-vis scanned from 200-800nm Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min. Only MeOH and Acetone samples (for both mannitol in tris and p-sorbitol in phosphate) were run today. The rest of the samples will be run tomorrow. p-Sorbitol in Phosphate Buffer (after sonication) Data UV-vis of p-Sorbitol solid in MeOH Fluorescence of p-Sorbitol solid in MeOH [[]] UV-vis of solid in Acetone Fluorescence of p-Sorbitol solid in Acetone [[]] Mannitol in Tris Buffer Data UV-vis of Mannitol solid in MeOH Fluorescence of Mannitol solid in MeOH [[]] UV-vis of Mannitol solid in Acetone Fluorescence of Mannitol solid in Acetone [[]]