User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/12 - Revision history

Dhea Patel at 14:53, 20 February 2013

Dhea Patel — Wed, 20 Feb 2013 14:53:30 GMT

←Older revision		Revision as of 14:53, 20 February 2013
Line 28:		Line 28:

	'''Only MeOH and Acetone samples (for both mannitol in tris and p-sorbitol in phosphate) were run today. The rest of the samples will be run [[User:Dhea_Patel/Notebook/Hemoglobin_Project/2013/02/13\|tomorrow]].'''		'''Only MeOH and Acetone samples (for both mannitol in tris and p-sorbitol in phosphate) were run today. The rest of the samples will be run [[User:Dhea_Patel/Notebook/Hemoglobin_Project/2013/02/13\|tomorrow]].'''
		+
		+	==p-Sorbitol in Phosphate Buffer (after sonication) Data==
		+
		+	*UV-vis of p-Sorbitol solid in MeOH
		+	[[Image:UV-vis_of_9.4mg_psorbitol_Phosphate_Buffer_Solid_in_1mL_MeOH_%28after_sonication%29_.png]]
		+	*Fluorescence of p-Sorbitol solid in MeOH
		+	[[]]
		+
		+
		+	*UV-vis of solid in Acetone
		+	[[Image:UV-vis_of_10.1mg_p-sorbiol_Phosphate_Buffer_Solid_in_1mL_Acetone_%28after_sonication%29_.png]]
		+	*Fluorescence of p-Sorbitol solid in Acetone
		+	[[]]
		+
		+
		+	==Mannitol in Tris Buffer Data==
		+
		+	*UV-vis of Mannitol solid in MeOH
		+	[[Image:UV-vis_of_11.7mg_Mannitol_Tris_Buffer_in_1mL_MeOH_.png]]
		+	* Fluorescence of Mannitol solid in MeOH
		+	[[]]
		+
		+	*UV-vis of Mannitol solid in Acetone
		+	[[Image:UV-vis_of_10.0mg_Mannitol_in_Tris_Buffer_Solid_in_1mL_Acetone.png]]
		+	* Fluorescence of Mannitol solid in Acetone
		+	[[]]
		+
		+

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Dhea Patel: /* Description */

Dhea Patel — Fri, 15 Feb 2013 14:50:30 GMT

Description

←Older revision		Revision as of 14:50, 15 February 2013
Line 10:		Line 10:

	==Description==		==Description==
		+
		+	*11.7mg mannitol in Tris buffer, solvent: MeOH
		+	*10.0mg mannitol in Tris buffer, solvent: Acetone
		+	*9.4mg p-sorbitol in phosphate buffer, solvent: MeOH
		+	*10.1mg p-sorbitol in phosphate buffer, solvent: Acetone
		+
	*all the mannitol in tris buffer samples were vortexed and centrifuged before being measured		*all the mannitol in tris buffer samples were vortexed and centrifuged before being measured
	*Centrifuge settings were as follows:		*Centrifuge settings were as follows:

Dhea Patel at 14:20, 13 February 2013

Dhea Patel — Wed, 13 Feb 2013 14:20:41 GMT

←Older revision		Revision as of 14:20, 13 February 2013
Line 6:		Line 6:
	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==Objective==
-	* ~~Insert content here..~~.	+	* Run UV-vis and Fluorescence on p-sorbitol in phosphate buffer and mannitol in tris buffer samples in either MeOH, acetone, acetonitrile, ethyl acetate, water, or chloroform.

		+	==Description==
		+	*all the mannitol in tris buffer samples were vortexed and centrifuged before being measured
		+	*Centrifuge settings were as follows:
		+	**13200rpm
		+	**0:16 seconds
		+	**851 rotor
		+	*all the p-sorbitol in phosphate buffer samples were sonicated for 3 hours in their respective solvents.
		+	*Each solvent was used as a blank.
		+	*The data was corrected for the blank and a corrected baseline.
		+	*UV-vis scanned from 200-800nm
		+	*Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min.
		+
		+	'''Only MeOH and Acetone samples (for both mannitol in tris and p-sorbitol in phosphate) were run today. The rest of the samples will be run [[User:Dhea_Patel/Notebook/Hemoglobin_Project/2013/02/13\|tomorrow]].'''

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Dhea Patel: Autocreate 2013/02/12 Entry for User:Dhea_Patel/Notebook/Hemoglobin_Project

Dhea Patel — Wed, 13 Feb 2013 14:13:52 GMT

Autocreate 2013/02/12 Entry for User:Dhea_Patel/Notebook/Hemoglobin_Project

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

__NOTOC__