User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/13

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==Entry title==
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==Objective==
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* Run UV-vis and Fluorescence on p-sorbitol in phosphate buffer and mannitol in tris buffer samples in either MeOH, acetone, acetonitrile, ethyl acetate, water, or chloroform.
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==Description==
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*Each solvent was used as a blank.
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*The data was corrected for the blank and a corrected baseline.
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*UV-vis scanned from 200-800nm
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*Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min.
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Revision as of 10:21, 13 February 2013

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Objective

  • Run UV-vis and Fluorescence on p-sorbitol in phosphate buffer and mannitol in tris buffer samples in either MeOH, acetone, acetonitrile, ethyl acetate, water, or chloroform.

Description

  • Each solvent was used as a blank.
  • The data was corrected for the blank and a corrected baseline.
  • UV-vis scanned from 200-800nm
  • Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min.



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