User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/13: Difference between revisions

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*UV-vis of Mannitol solid in Ethyl Acetate
*UV-vis of Mannitol solid in Ethyl Acetate
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[[Image:UV-vis_of_10.5mg_Mannitol_Tris_Buffer_in_1mL_Ethyl_Acetate_.png]]


*UV-vis of Mannitol solid in Water
*UV-vis of Mannitol solid in Water

Revision as of 08:23, 20 February 2013

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Objective

  • Run UV-vis and Fluorescence on p-sorbitol in phosphate buffer and mannitol in tris buffer samples in either MeOH, acetone, acetonitrile, ethyl acetate, water, or chloroform.

Description

  • 10.5mg mannitol in Tris buffer, solvent: Acetonitrile
  • 10.8mg p-sorbitol in phosphate buffer, solvent: Acetonitrile
  • 10.5mg mannitol in tris buffer, solvent: ethyl acetate
  • 12.1mg p-sorbitol in phosphate buffer, solvent: ethyl acetate
  • 11.7mg mannitol in tris buffer, solvent: water
  • 10.0mg p-sorbitol in phosphate buffer, solvent: water
  • 11.6mg mannitol in tris buffer, solvent: chloroform
  • 10.0mg p-sorbitol in phosphate buffer, solvent: chloroform
  • Each solvent was used as a blank.
  • The data was corrected for the blank and a corrected baseline.
  • UV-vis scanned from 200-800nm


p-Sorbitol in Phosphate Buffer (after sonication) Data

  • UV-vis of p-Sorbitol solid in Acetonitrile

  • UV-vis of p-Sorbitol solid in Ethyl Acetate

  • UV-vis of p-Sorbitol solid in Water

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  • UV-vis of p-Sorbitol solid in Chloroform

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Mannitol in Tris Buffer Data

  • UV-vis of Mannitol solid in Acetonitrile

  • UV-vis of Mannitol solid in Ethyl Acetate

  • UV-vis of Mannitol solid in Water

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  • UV-vis of Mannitol solid in Chloroform

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