User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/15: Difference between revisions

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==Entry title==
==Objective==
* Insert content here...
* Run Fluorescence on p-sorbitol in phosphate buffer and mannitol in tris buffer samples in either acetonitrile, ethyl acetate, water, or chloroform.


==Description==
*10.5mg mannitol in Tris buffer, solvent: Acetonitrile
*10.8mg p-sorbitol in phosphate buffer, solvent: Acetonitrile
*10.5mg mannitol in tris buffer, solvent: ethyl acetate
*12.1mg p-sorbitol in phosphate buffer, solvent: ethyl acetate
*11.7mg mannitol in tris buffer, solvent: water
*10.0mg p-sorbitol in phosphate buffer, solvent: water
*11.6mg mannitol in  tris buffer, solvent: chloroform
**centrifuged for an additional 10 minutes
*10.0mg p-sorbitol in phosphate buffer, solvent: chloroform
*all the mannitol in tris buffer samples were vortexed and centrifuged before being measured
*Centrifuge settings were as follows:
**13200rpm
**0:16 seconds
**851 rotor
*all the p-sorbitol in phosphate buffer samples were sonicated for 3 hours in their respective solvents.
*Each solvent was used as a blank.
*The data was corrected for the blank and a corrected baseline.
*Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min.


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Revision as of 07:58, 15 February 2013

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Objective

  • Run Fluorescence on p-sorbitol in phosphate buffer and mannitol in tris buffer samples in either acetonitrile, ethyl acetate, water, or chloroform.

Description

  • 10.5mg mannitol in Tris buffer, solvent: Acetonitrile
  • 10.8mg p-sorbitol in phosphate buffer, solvent: Acetonitrile
  • 10.5mg mannitol in tris buffer, solvent: ethyl acetate
  • 12.1mg p-sorbitol in phosphate buffer, solvent: ethyl acetate
  • 11.7mg mannitol in tris buffer, solvent: water
  • 10.0mg p-sorbitol in phosphate buffer, solvent: water
  • 11.6mg mannitol in tris buffer, solvent: chloroform
    • centrifuged for an additional 10 minutes
  • 10.0mg p-sorbitol in phosphate buffer, solvent: chloroform


  • all the mannitol in tris buffer samples were vortexed and centrifuged before being measured
  • Centrifuge settings were as follows:
    • 13200rpm
    • 0:16 seconds
    • 851 rotor
  • all the p-sorbitol in phosphate buffer samples were sonicated for 3 hours in their respective solvents.
  • Each solvent was used as a blank.
  • The data was corrected for the blank and a corrected baseline.
  • Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min.


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