Objective
- Run Fluorescence on p-sorbitol in phosphate buffer and mannitol in tris buffer samples in either acetonitrile, ethyl acetate, water, or chloroform.
Description
- 10.5mg mannitol in Tris buffer, solvent: Acetonitrile
- 10.8mg p-sorbitol in phosphate buffer, solvent: Acetonitrile
- 10.5mg mannitol in tris buffer, solvent: ethyl acetate
- 12.1mg p-sorbitol in phosphate buffer, solvent: ethyl acetate
- 11.7mg mannitol in tris buffer, solvent: water
- 10.0mg p-sorbitol in phosphate buffer, solvent: water
- 11.6mg mannitol in tris buffer, solvent: chloroform
- centrifuged for an additional 10 minutes
- 10.0mg p-sorbitol in phosphate buffer, solvent: chloroform
- all the mannitol in tris buffer samples were vortexed and centrifuged before being measured
- Centrifuge settings were as follows:
- 13200rpm
- 0:16 seconds
- 851 rotor
- all the p-sorbitol in phosphate buffer samples were sonicated for 3 hours in their respective solvents.
- Each solvent was used as a blank.
- The data was corrected for the blank and a corrected baseline.
- Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min.
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