User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/26: Difference between revisions
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== | ==Objective== | ||
* | *to run UV-vis on 11.6mg p-sorbitol in Citrate Buffer in MeOH at temperatures ranging from 5°C to 50°C, with 5°C increments. | ||
==Protocol== | |||
* MeOH blank was run in UV-vis and Fluorescence. | |||
* 11.6mg of myoglobin with KCl in 20mL 0.01M pH 3 citrate buffer (evaporated) made [[0.0058g Myoglobin + 0.4961g KCl in 20mL 0.01M pH 3 citrate buffer (evaporated)| January 30th]] was run in UV-vis and Fluorescence. | |||
*The data was analyzed and compared to the data from January 30th. To find that the UV-vis absorbance was the same, but the Fluorescence had a greater intensity. | |||
[[Image:UV-vis_of_11.6mg_Citrate_Solid_in_1mL_MeOH%2C_taken_February_26%2C_2013.png]] [[Image:UV-vis_of_11.6mg_Citrate_Solid_in_1mL_MeOH.png]] | |||
[[Image:Fluorescence_of_11.6mg_Citrate_Solid_in_1mL_MeOH%2C_taken_February_26%2C_2013.png]] [[Image:Fluorescence_of_11.6mg_Citrate_Solid_in_1mL_MeOH.png]] | |||
Revision as of 08:23, 26 February 2013
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