User:Dhea Patel/Notebook/Hemoglobin Project/2013/04/02

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Current revision (09:54, 2 April 2013) (view source)
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==Protocol==
==Protocol==
*15.2mL MeOH was added to 16.4mg o-Phenylenediamine.(10mM)
*15.2mL MeOH was added to 16.4mg o-Phenylenediamine.(10mM)
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*8.8mg Myoglobin, KCl in Citrate (pH3) was added to 1mL MeOH.
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*8.9mg Myoglobin, KCl in Citrate (pH3) was added to 1mL MeOH.
*1.2mL Hydrogen Peroxide was added to MeOH to make a 1M stock solution.
*1.2mL Hydrogen Peroxide was added to MeOH to make a 1M stock solution.

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Protocol

  • 15.2mL MeOH was added to 16.4mg o-Phenylenediamine.(10mM)
  • 8.9mg Myoglobin, KCl in Citrate (pH3) was added to 1mL MeOH.
  • 1.2mL Hydrogen Peroxide was added to MeOH to make a 1M stock solution.
  • UV-vis was run on the Mb in MeOH solution after it had been centrifuged to determine how much Mb was in solution.


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