User:Dhea Patel/Notebook/Hemoglobin Project/2013/06/13: Difference between revisions
Dhea Patel (talk | contribs) m (→Data) |
Dhea Patel (talk | contribs) m (→Data) |
||
| Line 24: | Line 24: | ||
The drop in absorbance at 120 minutes and 140 minutes is due to the outside of the cuvette being cleaned. | The drop in absorbance at 120 minutes and 140 minutes is due to the outside of the cuvette being cleaned. | ||
<b>Mb/Phosphate in Hexane</b> | |||
[[Image:Mb_Phosphate_OPD_H2O2_Hexane_WORKUP_GRAPH.bmp]] | |||
<b>Mb/Acetate in Hexane</b> | |||
[[Image:Mb_Acetate_OPD_H2O2_Hexane_WORKUP_GRAPH.bmp]] | |||
Note: The high absorbance peak at 140 minutes is due to a potential water/protein precipitate that filtered through. | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
Revision as of 13:17, 13 June 2013
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Reaction of OPD+H2O2 with Mb Catalyst in Acetone SuspensionThe procedurefor the reaction of OPD and H2O2 was followed using a Mb/Tris catalyst and an acetone suspension. The reaction was run in a beaker with a stir bar and the solution was centrifuged for 1 minute at 13,200 rpm before taking a spectrum. In addition reactions were run using Mb/Phosphate and Mb/Acetate in hexanes suspension. These samples were filtered (not centrifuged) before taking a spectrum. DataMb/Tris in Acetone, Trial 1
The drop in absorbance at 140 minutes is due to the outside of the cuvette being cleaned. Mb/Tris in Acetone, Trial 2
The drop in absorbance at 120 minutes and 140 minutes is due to the outside of the cuvette being cleaned. Mb/Phosphate in Hexane
Mb/Acetate in Hexane
Note: The high absorbance peak at 140 minutes is due to a potential water/protein precipitate that filtered through. | |
