User:Dhea Patel/Notebook/Hemoglobin Project/2013/06/13

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==Entry title==
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==Reaction of OPD+H<sub>2</sub>O<sub>2</sub> with Mb Catalyst in Acetone Suspension==
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The [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/Pauls_Notebook/2013/05/31 procedure]for the reaction of OPD and H<sub>2</sub>O<sub>2</sub> was followed using a Mb/Tris catalyst and an acetone suspension. The reaction was run in a beaker with a stir bar and the solution was centrifuged for 1 minute at 13,200 rpm before taking a spectrum.
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In addition reactions were run using Mb/Phosphate and Mb/Acetate in hexanes suspension.
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These samples were filtered (not centrifuged) before taking a spectrum.
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==Data==
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<b>Mb/Tris in Acetone, Trial 1</b>
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[[Image:Mb_Tris1_OPD_H2O2_Acetone_WORKUP_GRAPH.JPG]]
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The drop in absorbance at 140 minutes is due to the outside of the cuvette being cleaned.
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<b>Mb/Tris in Acetone, Trial 2</b>
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[[]]
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The drop in absorbance at 120 minutes and 140 minutes is due to the outside of the cuvette being cleaned.
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Revision as of 12:05, 13 June 2013

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Reaction of OPD+H2O2 with Mb Catalyst in Acetone Suspension

The procedurefor the reaction of OPD and H2O2 was followed using a Mb/Tris catalyst and an acetone suspension. The reaction was run in a beaker with a stir bar and the solution was centrifuged for 1 minute at 13,200 rpm before taking a spectrum.

In addition reactions were run using Mb/Phosphate and Mb/Acetate in hexanes suspension. These samples were filtered (not centrifuged) before taking a spectrum.

Data

Mb/Tris in Acetone, Trial 1 Image:Mb_Tris1_OPD_H2O2_Acetone_WORKUP_GRAPH.JPG

The drop in absorbance at 140 minutes is due to the outside of the cuvette being cleaned.

Mb/Tris in Acetone, Trial 2 [[]] The drop in absorbance at 120 minutes and 140 minutes is due to the outside of the cuvette being cleaned.


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