User:Dhea Patel/Notebook/Hemoglobin Project/2013/10/03: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(fix raw html notebook nav)
 
(One intermediate revision by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
Line 11: Line 11:
# Stock solutions:
# Stock solutions:
## Myoglobin
## Myoglobin
### 1.6mg (Mb:KCl lyophilized from Citrate, 5mg:500mg) in 1.0mL water
### 1.6mg (Mb:KCl lyophilized from Citrate, 5mg:500mg) in 1.0mL MeOH
## OPD
## OPD
### 4.1 mg OPD in 2.4mL water
### 4.1 mg OPD in 2.4mL MeOH
## H<sub>2</sub>O<sub>2</sub>
## H<sub>2</sub>O<sub>2</sub>
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL water
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL MeOH
#Experimental Run
#Experimental Run
## Sample chamber set to 5C and to stir
## Sample chamber set to 5C and to stir

Latest revision as of 23:24, 26 September 2017

Project name Main project page
Previous entry      Next entry

Procedure and Data

Mb in MeOH

  1. Stock solutions:
    1. Myoglobin
      1. 1.6mg (Mb:KCl lyophilized from Citrate, 5mg:500mg) in 1.0mL MeOH
    2. OPD
      1. 4.1 mg OPD in 2.4mL MeOH
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL MeOH
  2. Experimental Run
    1. Sample chamber set to 5C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 10 scan average
      4. Wait 1 minute after each scan
      5. Stop after 2 hours
    5. Files saved as: Mb_OPD_H2O2_Citrate_MeOH_25_SCANNUMBER.txt
    6. 0.225mL Mb stock solution added just before the third scan
    7. This is denoted in the data file for the third scan



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Dhea_Patel/Notebook/Hemoglobin_Project/2013/10/03&oldid=1013419"