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Project name
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Procedure and Data
Mb in MeOH
- Stock solutions:
- Myoglobin
- 1.8mg (Mb:KCl lyophilized from Citrate, 5mg:500mg) in 1.0mL MeOH
- OPD
- 4.1 mg OPD in 2.4mL MeOH
- H2O2
- 0.114mL 30% H2O2 in 0.887mL MeOH
- Experimental Run
- Sample chamber set to 35C and to stir
- N2 run through the cuvette holder to reduce condensation on cuvette
- 2.025mL OPD stock solution
- 0.75mL H2O2 stock solution
- Start Data Collection
- 1ms Integration
- 10 scan average
- Wait 1 minute after each scan
- Stop after 2 hours
- Files saved as: Mb_OPD_H2O2_Citrate_MeOH_25_SCANNUMBER.txt
- 0.225mL Mb stock solution added just before the third scan
- This is denoted in the data file for the third scan
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