User:Dhea Patel/Notebook/Hemoglobin Project/2013/10/04

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Procedure and Data

Mb in MeOH

  1. Stock solutions:
    1. Myoglobin
      1. 1.8mg (Mb:KCl lyophilized from Citrate, 5mg:500mg) in 1.0mL MeOH
    2. OPD
      1. 4.1 mg OPD in 2.4mL MeOH
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL MeOH
  2. Experimental Run
    1. Sample chamber set to 35C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 10 scan average
      4. Wait 1 minute after each scan
      5. Stop after 2 hours
    5. Files saved as: Mb_OPD_H2O2_Citrate_MeOH_35_SCANNUMBER.txt
    6. 0.225mL Mb stock solution added just before the third scan
    7. This is denoted in the data file for the third scan

Mb in MeOH

  1. Stock solutions:
    1. Myoglobin
      1. 1.8mg (Mb:KCl lyophilized from Citrate, 5mg:500mg) in 1.0mL MeOH
    2. OPD
      1. 4.1 mg OPD in 2.4mL MeOH
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL MeOH
  2. Experimental Run
    1. Sample chamber set to 45C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 10 scan average
      4. Wait 1 minute after each scan
      5. Stop after 2 hours
    5. Files saved as: Mb_OPD_H2O2_Citrate_MeOH_45_SCANNUMBER.txt
    6. 0.225mL Mb stock solution added just before the third scan
    7. This is denoted in the data file for the third scan



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