User:Dhea Patel/Notebook/Hemoglobin Project/2014/02/05: Difference between revisions
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== | ==OPD in H<sub>2</sub>O== | ||
Mb in MeOH | |||
#Stock solutions: | |||
##In Lieu of Myoglobin | |||
###1.0mL H<sub>2</sub>O was kept in a separate centrifuge tube. | |||
##OPD | |||
###3.8 mg OPD in 3.8mL H<sub>2</sub>O | |||
##H<sub>2</sub>O<sub>2</sub> | |||
###0.114mL 30% H2O2 in 0.887mL H<sub>2</sub>O | |||
#Experimental Run | |||
##Sample chamber set to 35C and to stir | |||
##2.025mL OPD stock solution | |||
##0.75mL H2O2 stock solution | |||
##Start Data Collection | |||
###1ms Integration | |||
###10 scan average | |||
###Wait 1 minute after each scan | |||
###Stop after 2 hours | |||
###Files saved as: OPD_H2O2_H2O_35_SCANNUMBER.txt | |||
##0.225mL H2O added just before the third scan | |||
###This is denoted in the data file for the third scan | |||
Revision as of 08:43, 5 February 2014
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OPD in H2OMb in MeOH
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