User:Dhea Patel/Notebook/Hemoglobin Project/2014/02/05: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Setup==
==Setting Up Ocean Optics==


Open Ocean Optics
*Run Wizard for Absorbance Spectroscopy
*Run Wizard for Absorbance Spectroscopy
*Configure Data Collection as described below
*Configure Data Collection as described below

Revision as of 09:25, 5 February 2014

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Setting Up Ocean Optics

  • Run Wizard for Absorbance Spectroscopy
  • Configure Data Collection as described below
  • Set Reference Blank and Dark
  • On the Absorbance view, click the "save" button to configure Save Settings
  • When you are ready to collect data, place the cuvette in the holder and click the "play" arrow followed by the "save" button.

OPD in H2O at 35C

  1. Stock solutions:
    1. In Lieu of Myoglobin
      1. 1.0mL H2O was kept in a separate centrifuge tube.
    2. OPD
      1. 3.8 mg OPD in 3.8mL H2O
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL H2O
  2. Experimental Run
    1. Sample chamber set to 35C and to stir
    2. 2.025mL OPD stock solution
    3. 0.75mL H2O2 stock solution
    4. Start Data Collection
      1. 1ms Integration
      2. 10 scan average
      3. Wait 1 minute after each scan
      4. Stop after 2 hours
      5. Files saved as: OPD_H2O2_H2O_35_SCANNUMBER.txt
    5. 0.225mL H2O added just before the third scan
      1. This is denoted in the data file for the third scan



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Dhea_Patel/Notebook/Hemoglobin_Project/2014/02/05&oldid=769267"