User:Dhea Patel/Notebook/Hemoglobin Project/2014/02/10

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Mb Phosphate & OPD in MeOH at 5C

  1. Stock solutions:
    1. Mb Phosphate
      1. 1.6mg Phosphate Mb was added to 1.0mL MeOH in a centrifuge tube.
    2. OPD
      1. 3.2 mg OPD in 3.2mL MeOH
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL MeOH
  2. Experimental Run
    1. Sample chamber set to 5C and to stir
    2. 2.025mL OPD stock solution
    3. 0.75mL H2O2 stock solution
    4. Start Data Collection
      1. 1ms Integration
      2. 10 scan average
      3. Wait 1 minute after each scan
      4. Stop after 2 hours
      5. Files saved as: Me_OPD_H2O2_Phosphate_MeOH_5_SCANNUMBER.txt
    5. 0.225mL Mb/MeOH added just before the third scan
      1. This is denoted in the data file for the third scan

Mb Phosphate & OPD in MeOH at 15C

  1. Stock solutions:
    1. Mb Phosphate
      1. The solution made above was used.
    2. OPD
      1. 3.6 mg OPD in 3.6mL MeOH
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL MeOH
  2. Experimental Run
    1. Sample chamber set to 5C and to stir
    2. 2.025mL OPD stock solution
    3. 0.75mL H2O2 stock solution
    4. Start Data Collection
      1. 1ms Integration
      2. 10 scan average
      3. Wait 1 minute after each scan
      4. Stop after 2 hours
      5. Files saved as: Me_OPD_H2O2_Phosphate_MeOH_15_SCANNUMBER.txt
    5. 0.225mL Mb/MeOH added just before the third scan
      1. This is denoted in the data file for the third scan



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