User:Dhea Patel/Notebook/Hemoglobin Project/2014/04/09: Difference between revisions
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== | ==Mb rxns at 25°C== | ||
#Stock solutions: | |||
##Mb Citrate | |||
###1.6mg Citrate Mb was added to 1.0mL MeOH in a centrifuge tube. | |||
##OPD | |||
###2.7 mg OPD in 2.7mL MeOH | |||
##H<sub>2</sub>O<sub>2</sub> | |||
###0.114mL 30% H2O2 in 0.887mL MeOH | |||
#Experimental Run | |||
##Sample chamber set to 25C and to stir | |||
##2.025mL OPD stock solution | |||
##0.75mL H<sub>2</sub>O<sub>2</sub> stock solution | |||
##Start Data Collection | |||
###1ms Integration | |||
###10 scan average | |||
###Wait 1 minute after each scan | |||
###Stop after 2 hours | |||
###Files saved as: Me_OPD_H2O2_Phosphate_MeOH_5_SCANNUMBER.txt | |||
##0.225mL Mb/MeOH added just before the third scan | |||
###This is denoted in the data file for the third scan | |||
Paul will make 1L phosphate buffer @ same pH as last week. He will also make 1L of phosphate buffer with 1 molar NaCl. | |||
Revision as of 06:58, 9 April 2014
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Mb rxns at 25°C
Paul will make 1L phosphate buffer @ same pH as last week. He will also make 1L of phosphate buffer with 1 molar NaCl.
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