User:Dhea Patel/Notebook/Hemoglobin Project/2014/04/21: Difference between revisions

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#Stock solutions:
#Stock solutions:
##Mb Tris
##Mb Tris
###1.6mg Tris Mb was added to 1.0mL H2O in a centrifuge tube.  
###2.0mg Tris Mb was added to 1.0mL H2O in a centrifuge tube.  
##OPD
##OPD
###2.7 mg OPD in 2.7mL H2O  
###3.5 mg OPD in 3.5mL H2O  
##H<sub>2</sub>O<sub>2</sub>
##H<sub>2</sub>O<sub>2</sub>
###0.114mL 30% H2O2 in 0.887mL H2O  
###0.114mL 30% H2O2 in 0.887mL H2O  

Revision as of 07:12, 21 April 2014

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Mb rxns at 25°C

  1. Stock solutions:
    1. Mb Tris
      1. 2.0mg Tris Mb was added to 1.0mL H2O in a centrifuge tube.
    2. OPD
      1. 3.5 mg OPD in 3.5mL H2O
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL H2O
  2. Experimental Run
    1. Sample chamber set to 25C and to stir
    2. 2.025mL OPD stock solution
    3. 0.75mL H2O2 stock solution
    4. Start Data Collection
      1. 1ms Integration
      2. 10 scan average
      3. Wait 1 minute after each scan
      4. Stop after 2 hours
      5. Files saved as: Mb_Tris_OPD_H2O2_H2O_25_SCANNUMBER.txt
    5. 0.225mL Mb/H2O added just before the third scan
      1. This is denoted in the data file for the third scan


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