User:Dhea Patel/Notebook/Phosphorylation/2012/06/06: Difference between revisions
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'''Fractions 4-6''' | '''Fractions 4-6''' | ||
Ru complex+paper: 0.4826g | |||
Ru complex+paper: 0.4826g | |||
Weigh Paper: 0.2662g | Weigh Paper: 0.2662g | ||
''Ru complex: 0.2164g'' | ''Ru complex: 0.2164g'' | ||
'''Fractions 7''' | '''Fractions 7''' | ||
Ru complex+paper: 0.3234g | |||
Ru complex+paper: 0.3234g | |||
Weigh Paper: 0.2612g | Weigh Paper: 0.2612g | ||
''Ru complex: 0.0622g'' | ''Ru complex: 0.0622g'' | ||
'''Fractions and Observations''' | |||
Fraction 4-6: Ru comp: 0.2164g | filtered clear liquid | |||
Fraction 7: Ru comp: 0.0622g | filtered clear liquid | |||
Fraction 8: Ru comp: 0.0028g | filtered yellow liquid | |||
Fraction 1: Ru comp: 0.0213g | filtered yellow liquid | |||
Fraction 2: Ru comp: 0.0219g | | |||
Fraction 3: Ru comp: 0.0011g | | |||
Fraction 9: Ru comp: 0.0004g | | |||
Fraction 10: Ru comp: 0.0039g | | |||
==Calculations== | ==Calculations== | ||
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5E-5 mol ATP × 507.18 g/mol = '''''0.02445 g ATP''''' | 5E-5 mol ATP × 507.18 g/mol = '''''0.02445 g ATP''''' | ||
'''Predicting product mass''' | |||
RuComplex: RuC<sub>32</sub>H<sub>25</sub>N<sub>7</sub> P<sub>2</sub>F<sub>12</sub> | |||
ATP: C<sub>10</sub>H<sub>12</sub>N<sub>5</sub> O<sub>12</sub>P<sub>3</sub> | |||
RuATP: RuC<sub>42</sub>H<sub>37</sub>N<sub>12</sub>O<sub>12</sub>P<sub>5</sub>F<sub>12</sub> | |||
Ru - 101.17 g/mol | |||
C - 504.42 g/mol | |||
H - 37.37 g/mol | |||
N - 168.12 g/mol | |||
O - 192 g/mol | |||
P - 154.85 g/mol | |||
F - 228 g/mol | |||
'''''Total - 1385.83 g/mol''''' | |||
==Notes== | ==Notes== | ||
This area is for any observations or conclusions that you would like to note. | This area is for any observations or conclusions that you would like to note. |
Revision as of 08:01, 25 July 2012
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ObjectiveLearn how to maintain an OpenWetWare Notebook. Description
DataFractions 4-6 Ru complex+paper: 0.4826g Weigh Paper: 0.2662g Ru complex: 0.2164g Fractions 7 Ru complex+paper: 0.3234g Weigh Paper: 0.2612g Ru complex: 0.0622g Fractions and Observations Fraction 4-6: Ru comp: 0.2164g | filtered clear liquid Fraction 7: Ru comp: 0.0622g | filtered clear liquid Fraction 8: Ru comp: 0.0028g | filtered yellow liquid Fraction 1: Ru comp: 0.0213g | filtered yellow liquid Fraction 2: Ru comp: 0.0219g | Fraction 3: Ru comp: 0.0011g | Fraction 9: Ru comp: 0.0004g | Fraction 10: Ru comp: 0.0039g | Calculations0.2164g ÷ (898.65g/mol) = 2.5E-4 mol --> 0.25 mmol Ru complex. 0.25 mmol Ru complex ÷ 5 equivalents = 0.05 mmol ATP ATMP solution: 0.18 mmol × 5 equivalents = 0.9 mmol --> 1 mmol 5E-5 mol ATP × 507.18 g/mol = 0.02445 g ATP
RuComplex: RuC32H25N7 P2F12 ATP: C10H12N5 O12P3 RuATP: RuC42H37N12O12P5F12
Ru - 101.17 g/mol C - 504.42 g/mol H - 37.37 g/mol N - 168.12 g/mol O - 192 g/mol P - 154.85 g/mol F - 228 g/mol Total - 1385.83 g/mol NotesThis area is for any observations or conclusions that you would like to note. |