User:Dhea Patel/Notebook/Phosphorylation/2012/06/06: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(2 intermediate revisions by the same user not shown)
Line 50: Line 50:


5E-5 mol ATP × 507.18 g/mol = '''''0.02445 g ATP'''''
5E-5 mol ATP × 507.18 g/mol = '''''0.02445 g ATP'''''
'''Predicting product mass'''
RuComplex: RuC<sub>32</sub>H<sub>25</sub>N<sub>7</sub>                                P<sub>2</sub>F<sub>12</sub>
ATP:                C<sub>10</sub>H<sub>12</sub>N<sub>5</sub>  O<sub>12</sub>P<sub>3</sub>
RuATP:        RuC<sub>42</sub>H<sub>37</sub>N<sub>12</sub>O<sub>12</sub>P<sub>5</sub>F<sub>12</sub>
Ru - 101.17 g/mol
  C - 504.42 g/mol
  H - 37.37  g/mol
  N - 168.12 g/mol
  O - 192    g/mol
  P - 154.85 g/mol
  F - 228    g/mol
'''''Total - 1385.83 g/mol'''''


==Notes==
==Notes==
This area is for any observations or conclusions that you would like to note.
This area is for any observations or conclusions that you would like to note.

Revision as of 08:01, 25 July 2012

Phosphorylation Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>



Objective

Learn how to maintain an OpenWetWare Notebook.

Description

  1. Add experimental record here. Include what, how, and why...

Data

Fractions 4-6

     Ru complex+paper: 0.4826g
          Weigh Paper: 0.2662g
           Ru complex: 0.2164g

Fractions 7

     Ru complex+paper: 0.3234g
          Weigh Paper: 0.2612g
           Ru complex: 0.0622g

Fractions and Observations

  Fraction 4-6: Ru comp: 0.2164g | filtered clear liquid
    Fraction 7: Ru comp: 0.0622g | filtered clear liquid
    Fraction 8: Ru comp: 0.0028g | filtered yellow liquid
    Fraction 1: Ru comp: 0.0213g | filtered yellow liquid
    Fraction 2: Ru comp: 0.0219g |
    Fraction 3: Ru comp: 0.0011g |
    Fraction 9: Ru comp: 0.0004g |
   Fraction 10: Ru comp: 0.0039g |

Calculations

0.2164g ÷ (898.65g/mol) = 2.5E-4 mol --> 0.25 mmol Ru complex.

0.25 mmol Ru complex ÷ 5 equivalents = 0.05 mmol ATP

ATMP solution: 0.18 mmol × 5 equivalents = 0.9 mmol --> 1 mmol

5E-5 mol ATP × 507.18 g/mol = 0.02445 g ATP


Predicting product mass

RuComplex: RuC32H25N7 P2F12

ATP: C10H12N5 O12P3

RuATP: RuC42H37N12O12P5F12


Ru - 101.17 g/mol
  C - 504.42 g/mol
  H - 37.37  g/mol
  N - 168.12 g/mol
  O - 192    g/mol
  P - 154.85 g/mol
  F - 228    g/mol
Total - 1385.83 g/mol

Notes

This area is for any observations or conclusions that you would like to note.