User:Dhea Patel/Notebook/Phosphorylation/2012/06/22: Difference between revisions
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==Description== | ==Description== | ||
# | # The mixture was filtered under the hood. Clear crystals were collected. | ||
# The filtered solution was run in the fast-flowing DEAE column (which had run 100mL H<sub>2</sub>O immediately before). A small orange band formed at the top of the column. | |||
#After the sample was added, 125mL of H<sub>2</sub>O was run. | |||
#This was followed by 60mL 0.1M TEAB. This gradient continued until the column cleared. (Gradient: H<sub>2</sub>O, 0.1M TEAB, 0.2 M TEAB, 0.3 M TEAB, 0.4 M TEAB, 0.5 M TEAB, 0.6 M TEAB, 1 M TEAB). | |||
#200mL of 1M TEAB was run | |||
#150mL of 1 M TEAB : 1 M NaCl | |||
#300mL of H<sub>2</sub>O (to clean out the column) | |||
==Observations== | |||
Mixture after filtering: opaque solution resembling orange juice. | |||
After the mixture was added to the column, the liquid that was leaving the column as the sample was going into the column material was cloudy white. After adding more water to the column the run-through was cloudy until the water added to the column was clear. The run-throughs remained clear through the TEAB gradient. An orange band did form in the column and began to move slowly with 0.2 M TEAB. | |||
==Data== | ==Data== | ||
Revision as of 10:32, 8 March 2013
 Phosphorylation Lab
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ObjectiveLearn how to maintain an OpenWetWare Notebook. Description
ObservationsMixture after filtering: opaque solution resembling orange juice. After the mixture was added to the column, the liquid that was leaving the column as the sample was going into the column material was cloudy white. After adding more water to the column the run-through was cloudy until the water added to the column was clear. The run-throughs remained clear through the TEAB gradient. An orange band did form in the column and began to move slowly with 0.2 M TEAB. Data
NotesThis area is for any observations or conclusions that you would like to note. |
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