User:Dhea Patel/Notebook/Phosphorylation/2012/07/24: Difference between revisions
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#Purified ATP was rotovapped with 10mL MeOH three times. | #Purified ATP was rotovapped with 10mL MeOH three times. | ||
#1.8mL DMF was added to the ATP under nitrogen. 0. | #1.8mL DMF was added to the ATP under nitrogen. 0.0301g DCC was added to the reaction mixture and allowed to react for 3 hours. | ||
#0.048805g (1,10)-Phenanthrolin-5-amine was dissolved in 20mL MeOH and added to the reaction mixture, along with 0.25mL TEA and allowed to react for 30 minutes. | #0.048805g (1,10)-Phenanthrolin-5-amine was dissolved in 20mL MeOH and added to the reaction mixture, along with 0.25mL TEA and allowed to react for 30 minutes. | ||
#The reaction was quenched in 40mL H<sub>2</sub>O. | #The reaction was quenched in 40mL H<sub>2</sub>O. |
Revision as of 06:50, 26 September 2012
Phosphorylation Lab | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveLearn how to maintain an OpenWetWare Notebook. Description
Calculations0.25mmol (1,10)-Phenantolin-5-amine 0.25mmol ÷ 1000mmol/mol × 195.22g/mol = 0.048805g Data
NotesThe crystal growth systems were observed. It appeared that the methanol/chloroform system began growing crystals. A seventh system was set up using a larger RuATP sample and minimal methanol. |